The correlation between expression and localization of a foreign gene product in rice endosperm

Plant & Cell Physiology
Hiroshi YasudaFumio Takaiwa

Abstract

Glucagon-like peptide 1 (GLP-1) is a 30 amino acid peptide hormone involved in insulin stimulation that is dependent upon blood glucose levels. We have previously reported that when this short peptide gene was directly expressed under the control of a glutelin promoter and its signal peptide, it was not accumulated in transgenic rice seed due to gene silencing. However, when the modified GLP-1 (mGLP-1) gene was enlarged to 5xmGLP-1 (mGLPx5) by tandem repeat, no silencing was observed. The mGLPx5 peptide could be accumulated in rice seed and its localization was mainly limited to the endoplasmic reticulum (ER). We also investigated alternative cellular localization sites that would increase accumulation. The relationship between the expression level and localization was examined by attaching the chitinase signal peptide to mGLPx5 to direct it into the intercellular space (apoplast), or by expression as a fusion protein with glutelin by insertion into a variable region of the acidic subunit, thus directing the peptide to protein body II (PB II). Attachment of the KDEL ER retention signal to the 6xmGLP-1 (mGLPx6) or its fusion to the C-terminus of the 13 kDa prolamin directed the peptide to the ER or PB I, respectively. Unexpected...Continue Reading

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Citations

Feb 2, 2013·Plant Molecular Biology·Kyoko KudoFumio Takaiwa
Aug 25, 2011·Journal of Agricultural and Food Chemistry·Tadayoshi HiraiHiroshi Ezura
Apr 24, 2014·BMC Plant Biology·Mireia BundóMaría Coca
Oct 10, 2012·Journal of Plant Physiology·Yuhya WakasaFumio Takaiwa
Jan 18, 2011·Biotechnology Advances·Jianfeng XuMaureen C Dolan
May 23, 2009·Biotechnology Advances·On Sun Lau, Samuel S M Sun
Apr 20, 2021·Frontiers in Plant Science·Ai SasouHiroshi Kiyono

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