The correlation between specific protein synthesis and tumoricidal function in murine peritoneal macrophages

Cellular Immunology
C S Tannenbaum, M T Largen

Abstract

Macrophages from the lipopolysaccharide (LPS)-responsive C3H/HeN mouse strain and the closely related LPS-nonresponsive C3H/HeJ strain were compared for tumoricidal activation and protein synthetic changes following in vivo and in vitro stimulation, utilizing two-dimensional polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins. Peritoneal macrophages elicited from C3H/HeN mice with heat-killed Propionibacterium acnes exhibited tumoricidal activity in a 16-hr cytolytic assay and expressed cytoplasmic levels of a 23.5-kDa protein during 48 hr of culture. The inability to detect persistent expression of p23.5 in P. acnes-stimulated C3H/HeJ macrophages correlated with the cytolytic impotence of those cells in the 16-hr chromium release assay. C3H/HeN macrophage populations lacking tumoricidal capacity could be rendered lytic, as could P. acnes-elicited C3H/HeJ macrophages, following in vitro stimulation with bacterial lipopolysaccharide. Concomitant with the LPS-induced expression of new functional activity was the appearance of augmented levels of several macrophage-specific proteins, including p23.5. This effect was dependent upon the lipid A moiety of LPS as the effects of LPS could be blocked by inclusion of p...Continue Reading

References

Apr 1, 1979·European Journal of Immunology·T SpringerC Milstein
Jul 15, 1977·Science·J B HibbsJ B Weinberg
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jun 1, 1987·Journal of Leukocyte Biology·C S TannenbaumM T Largen
Jan 1, 1984·Annual Review of Immunology·D O Adams, T A Hamilton

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