The cotton MAPK kinase GhMPK20 negatively regulates resistance to Fusarium oxysporum by mediating the MKK4-MPK20-WRKY40 cascade

Molecular Plant Pathology
Chen WangXingqi Guo

Abstract

Fusarium wilt is one of the most serious diseases affecting cotton. However, the pathogenesis and mechanism by which Fusarium oxysporum overcomes plant defence responses are unclear. Here, a new group D mitogen-activated protein kinase (MAPK) gene, GhMPK20, was identified and functionally analysed in cotton. GhMPK20 expression was significantly induced by F. oxysporum. Virus-induced gene silencing (VIGS) of GhMPK20 in cotton increased the tolerance to F. oxysporum, whereas ectopic GhMPK20 overexpression in Nicotiana benthamiana reduced F. oxysporum resistance via disruption of the salicylic acid (SA)-mediated defence pathway. More importantly, an F. oxysporum-induced MAPK cascade pathway composed of GhMKK4, GhMPK20 and GhWRKY40 was identified. VIGS of GhMKK4 and GhWRKY40 also enhanced F. oxysporum resistance in cotton, and the function of GhMKK4-GhMPK20 was shown to be essential for F. oxysporum-induced GhWRKY40 expression. Together, our results indicate that the GhMKK4-GhMPK20-GhWRKY40 cascade in cotton plays an important role in the pathogenesis of F. oxysporum. This research broadens our knowledge of the negative role of the MAPK cascade in disease resistance in cotton and provides an important scientific basis for the formu...Continue Reading

References

Nov 18, 1997·Cell·R Medzhitov, C A Janeway
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·J P Morrissey, A E Osbourn
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Y YangS Zhang
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·T P DelaneyJ A Ryals
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Jul 18, 2002·Trends in Plant Science·UNKNOWN MAPK Group
Jul 8, 2003·Current Genetics·Manuel D Ospina-GiraldoSeogchan Kang
Jan 22, 2004·Current Opinion in Immunology·David A Jones, Daigo Takemoto
Sep 17, 2004·The Journal of Biological Chemistry·Kerry F Pedley, Gregory B Martin
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·M RepB J C Cornelissen
Aug 5, 2005·Annual Review of Phytopathology·Jane Glazebrook
Dec 20, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C WangP A Roberts
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Lisa M SchechterAlan Collmer
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Nov 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ganesan SunilkumarKeerti S Rathore
Apr 28, 2009·The Plant Journal : for Cell and Molecular Biology·Ankit WaliaBrian Ellis
Apr 30, 2009·Annual Review of Phytopathology·A Corina VlotDaniel F Klessig
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
May 6, 2010·Annual Review of Plant Biology·Maria Cristina Suarez RodriguezJohn Mundy
Jun 8, 2010·The Plant Journal : for Cell and Molecular Biology·Mitsuko Kishi-KaboshiHirohiko Hirochika
Sep 1, 2003·Molecular Plant Pathology·Antonio Di PietroM Isabel G Roncero
Mar 20, 2012·Current Opinion in Plant Biology·Nobuaki Ishihama, Hirofumi Yoshioka
Apr 3, 2012·Current Opinion in Plant Biology·Feng Feng, Jian-Min Zhou
Apr 14, 2012·Trends in Plant Science·Jennifer S ThalerNoah K Whiteman
May 15, 2013·Annual Review of Phytopathology·Xiangzong Meng, Shuqun Zhang
Aug 28, 2013·G3 : Genes - Genomes - Genetics·Justin T PageJoshua A Udall
Feb 14, 2014·Plant Biotechnology Journal·Zhouhang GuXueping Zhou
Jun 20, 2014·Molecular Genetics and Genomics : MGG·Lingling DouShuxun Yu

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Citations

Apr 27, 2018·Frontiers in Plant Science·Jean Bigeard, Heribert Hirt
Nov 13, 2020·Plant Science : an International Journal of Experimental Plant Biology·Mengyu LiuZhonghai Ren
Jan 19, 2022·EMBO Reports·Tongjun Sun, Yuelin Zhang

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