The coumarin derivative AD6 inhibits the release of arachidonic acid by interfering with phospholipase A2 activity in human platelets stimulated with thrombin

Agents and Actions
S PorcellatiG Goracci

Abstract

AD6 is a coumarin derivative which is able to inhibit platelet aggregation and release due to various agonists as adrenaline, PAF, Ca++ ionophore and others. It has been demonstrated that this compound reduces the production of free arachidonate and diglyceride from human platelets pulse-labeled with radioactive arachidonic acid thus suggesting a possible interference with the activity of phospholipase A2 and/or phospholipase C. The present report indicates that the drug has no effect on the increase of the labeling of phosphatidic acid which takes place when platelets pulse-labeled with arachidonic acid are stimulated with thrombin. Furthermore, AD6 is not able to cause changes on the metabolism of phosphoinositides monitored using platelets pre-labeled with [3H] inositol. These observations exclude the possibility that AD6 interferes with phospholipase C activity. Experiments with platelets pulse-labeled with arachidonate suggest that AD6 inhibits phospholipase(s) A2 activity or modulate negatively one or more processes involved in its activation.

References

Apr 1, 1979·The Journal of Clinical Investigation·S Rittenhouse-Simmons
Sep 12, 1979·Biochemical and Biophysical Research Communications·M M BillahP Cuatrecasas
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L BellP W Majerus
Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
May 3, 1976·Biochemical and Biophysical Research Communications·S Rittenhouse-SimmonsD Deykin
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A P TruettR Snyderman
Mar 1, 1987·Thrombosis Research·R MozziG Porcellati
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S E Rittenhouse
Nov 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R L Kinlough-RathboneJ F Mustard

❮ Previous
Next ❯

Citations

Nov 28, 2002·Pharmacology & Therapeutics·Bent H Havsteen
Feb 1, 1994·British Journal of Pharmacology·S TranchinaA Del Maschio
Jul 6, 2004·The Journal of Pharmacy and Pharmacology·Claudio BucoloRosario Pignatello
Jun 30, 2006·The Journal of Pharmacy and Pharmacology·R PignatelloC Bucolo
Jun 3, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Dong-Ju SonYeo-Pyo Yun
Dec 3, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P N R Nair

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.