The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells

International Journal of Oncology
Yifei WangNicholi Vorsa

Abstract

Cranberry flavonoids (flavonols and flavan-3-ols), in addition to their antioxidant properties, have been shown to possess potential in vitro activity against several cancers. However, the difficulty of isolating cranberry compounds has largely limited anticancer research to crude fractions without well-defined compound composition. In this study, individual cranberry flavonoids were isolated to the highest purity achieved so far using gravity and high performance column chromatography and LC-MS characterization. MTS assay indicated differential cell viability reduction of SKOV-3 and OVCAR-8 ovarian cancer cells treated with individual cranberry flavonoids. Treatment with quercetin aglycone and PAC DP-9, which exhibited the strongest activity, induced apoptosis, led to caspase-3 activation and PARP deactivation, and increased sensitivity to cisplatin. Furthermore, immunofluorescence microscopy and western blot study revealed reduced expression and activation of epidermal growth factor receptor (EGFR) in PAC DP-9 treated SKOV-3 cells. In addition, quercetin aglycone and PAC DP-9 deactivated MAPK-ERK pathway, induced downregulation of cyclin D1, DNA-PK, phospho-histone H3 and upregulation of p21, and arrested cell cycle progressi...Continue Reading

References

Mar 15, 1990·International Journal of Cancer. Journal International Du Cancer·R J SchilderT C Hamilton
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L AgarwalG R Stark
Jun 23, 2001·Oncogene·F Hans, S Dimitrov
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y Yarden
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R I NicholsonM E Harper
Dec 26, 2001·Chemical Reviews·E R Jamieson, S J Lippard
Jun 20, 2003·Cell Proliferation·Katrien VermeulenZwi N Berneman
Jul 2, 2003·Nature Reviews. Cancer·Roshan Agarwal, Stan B Kaye
Jan 22, 2004·Journal of Agricultural and Food Chemistry·Irina O VvedenskayaNicholi Vorsa
Feb 7, 2004·Molecular & Cellular Proteomics : MCP·Ian J Jacobs, Usha Menon
Jun 3, 2004·The Journal of Nutrition·Peter J FergusonD James Koropatnick
Dec 22, 2006·The Journal of Nutrition·Catherine C Neto
Apr 26, 2007·Chemical Reviews·Yongwon Jung, Stephen J Lippard
Dec 7, 2007·International Journal of Radiation Biology·H Peter RodemannMahmoud Toulany
Mar 26, 2008·Journal of Medicinal Food·Ted WilsonFrances R Ragsdale
Oct 29, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shao-Chun Wang, Mien-Chie Hung
Feb 18, 2010·Food Chemistry·Ajay P SinghNicholi Vorsa

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Citations

Feb 10, 2016·BioMed Research International·Chen-Yi LiaoWu-Hsien Kuo
Nov 17, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yang WangQing-Yu He
Oct 4, 2016·Journal of Agricultural and Food Chemistry·Yifei WangNicholi Vorsa
Feb 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mario J SimirgiotisBeatriz Sepúlveda
Jul 25, 2018·Phytotherapy Research : PTR·Abdur RaufMohammad S Mubarak
Jan 10, 2018·Journal of the Science of Food and Agriculture·Shaomin ZhaoLiwei Gu
Apr 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haizhi HuangYi Charlie Chen
Jun 11, 2020·Archives of Physiology and Biochemistry·Masoud NajafiMilad Ashrafizadeh
Jan 18, 2017·Nutrition and Cancer·Wissam Zam, Aziz Khadour
Mar 31, 2016·Ukrainian biochemical journal·A A Philchenkov, M P Zavelevych
Jul 6, 2019·Anti-cancer Agents in Medicinal Chemistry·Maria MantzorouConstantinos Giaginis
Jun 20, 2020·Phytotherapy Research : PTR·Zahra RashidiFardin Amidi
Dec 23, 2016·Medicinal Research Reviews·Yuee CaiMeiwan Chen
Aug 31, 2020·Journal of Food Composition and Analysis : an Official Publication of the United Nations University, International Network of Food Data Systems·Yifei WangPei Chen
Sep 18, 2020·Protein and Peptide Letters·Loganayaki PeriyasamySridhar Muthusami

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Methods Mentioned

BETA
column chromatography
protein assay
electrophoresis
nuclear translocation
co-immunoprecipitation
Co-IP
flow cytometry

Software Mentioned

SPOT Advanced
SPSS Statistics
ModFit LT

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