The cricket paralysis virus suppressor inhibits microRNA silencing mediated by the Drosophila Argonaute-2 protein

PloS One
Corinne Besnard-GuérinChristophe Antoniewsk

Abstract

Small RNAs are potent regulators of gene expression. They also act in defense pathways against invading nucleic acids such as transposable elements or viruses. To counteract these defenses, viruses have evolved viral suppressors of RNA silencing (VSRs). Plant viruses encoded VSRs interfere with siRNAs or miRNAs by targeting common mediators of these two pathways. In contrast, VSRs identified in insect viruses to date only interfere with the siRNA pathway whose effector Argonaute protein is Argonaute-2 (Ago-2). Although a majority of Drosophila miRNAs exerts their silencing activity through their loading into the Argonaute-1 protein, recent studies highlighted that a fraction of miRNAs can be loaded into Ago-2, thus acting as siRNAs. In light of these recent findings, we re-examined the role of insect VSRs on Ago-2-mediated miRNA silencing in Drosophila melanogaster. Using specific reporter systems in cultured Schneider-2 cells and transgenic flies, we showed here that the Cricket Paralysis virus VSR CrPV1-A but not the Flock House virus B2 VSR abolishes silencing by miRNAs loaded into the Ago-2 protein. Thus, our results provide the first evidence that insect VSR have the potential to directly interfere with the miRNA silencing...Continue Reading

References

Dec 31, 2003·Cell·Jeffrey M VargasonTraci M Tanka Hall
Jul 3, 2004·Genes & Development·Katsutomo OkamuraMikiko C Siomi
May 28, 2005·Journal of Virology·Christopher S Sullivan, Don Ganem
Aug 9, 2005·Current Biology : CB·Philipp J F LeuschnerJavier Martinez
Oct 18, 2005·Nature Structural & Molecular Biology·Jeffrey A ChaoJames R Williamson
Nov 17, 2005·Genes & Development·Keita MiyoshiMikiko C Siomi
Mar 24, 2006·Nature Immunology·Delphine Galiana-ArnouxJean-Luc Imler
May 26, 2006·The EMBO Journal·Lóránt LakatosJózsef Burgyán
Sep 12, 2006·The Journal of General Virology·Mazen S HabayebDan Hultmark
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Jul 31, 2007·Cell·Yukihide TomariPhillip D Zamore
Aug 19, 2007·Cell·Shou-Wei Ding, Olivier Voinnet
Jul 5, 2008·Developmental Biology·Benjamin HaleyMichael Levine
Sep 30, 2008·Fly·Yu-ting ChouEric C Lai
Feb 6, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gatikrushna SinghRaj K Bhatnagar
Feb 26, 2009·Cell·Olivier Voinnet
Jun 16, 2009·Seminars in Cell & Developmental Biology·Veria Alvarado, Herman B Scholthof
Nov 18, 2009·Molecular Cell·Katsutomo OkamuraEric C Lai
Nov 18, 2009·Molecular Cell·Benjamin CzechGregory J Hannon
Apr 20, 2010·Nature Structural & Molecular Biology·Arabinda NayakRaul Andino
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Benjamin HaleyMichael Levine
Apr 5, 2011·Nature Methods·Jian-Quan NiNorbert Perrimon
Aug 24, 2012·PLoS Pathogens·Joël T van MierloRonald P van Rij
Apr 5, 2013·Immunological Reviews·Esmerina TiliCarlo M Croce
Jun 27, 2013·Nature Reviews. Molecular Cell Biology·Stefan L Ameres, Phillip D Zamore
Aug 13, 2013·Journal of Invertebrate Pathology·Diveena VijayendranBryony C Bonning
Oct 12, 2013·Science·P V MaillardOlivier Voinnet

❮ Previous
Next ❯

Citations

Mar 1, 2016·Scientific Reports·Jimena Carrillo-TrippBryony C Bonning
Jan 17, 2020·Nucleic Acids Research·Margarita T AngelovaClément Carré

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockdown
transgenic
biosensor
biosensors
transfection
transfections

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.