The CRISPR/Cas9 System for Targeted Genome Engineering in Free-Living Fungi: Advances and Opportunities for Lichenized Fungi

Frontiers in Microbiology
Karthik ShanmugamG N Hariharan

Abstract

Studies using whole genome sequencing, computational and gene expression, targeted genome engineering techniques for generating site-specific sequence alterations through non-homologous end joining (NHEJ) by genomic double-strand break (DSB) repair pathway with high precision, resulting in gene inactivation have elucidated the complexity of gene expression, and metabolic pathways in fungi. These tools and the data generated are crucial for precise generation of fungal products such as enzymes, secondary metabolites, antibiotics etc. Artificially engineered molecular scissors, zinc finger nucleases (ZFNs), Transcriptional activator-like effector nucleases (TALENs; that use protein motifs for DNA sequence recognition in the genome) and CRISPR associated protein 9 (Cas9;CRISPR/Cas9) system (RNA-DNA recognition) are being used in achieving targeted genome modifications for modifying traits in free-living fungal systems. Here, we discuss the recent research breakthroughs and developments which utilize CRISPR/Cas9 in the metabolic engineering of free-living fungi for the biosynthesis of secondary metabolites, enzyme production, antibiotics and to develop resistance against post-harvest browning of edible mushrooms and fungal pathogen...Continue Reading

References

Nov 26, 1999·Applied Microbiology and Biotechnology·A L Demain
Aug 14, 2001·Applied Microbiology and Biotechnology·K Müller
Apr 16, 2002·Molecular Microbiology·Ruud JansenLeo M Schouls
Aug 5, 2003·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Jose L Adrio, Arnold L Demain
Mar 29, 2005·Journal of Molecular Evolution·Francisco J M MojicaElena Soria
Feb 10, 2006·Cell Research·Richard J WeldHayley J Ridgway
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Jan 29, 2010·Applied Microbiology and Biotechnology·Ulrich Kück, Birgit Hoff
Dec 7, 2010·Nature Structural & Molecular Biology·Yu Zhang, Maria Jasin
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 5, 2013·Science·Prashant MaliGeorge M Church
Jan 31, 2013·Nature Biotechnology·Wenyan JiangLuciano A Marraffini
Mar 6, 2013·Nucleic Acids Research·James E DiCarloGeorge M Church
Mar 4, 2014·Nature Biotechnology·Jeffry D Sander, J Keith Joung
Oct 30, 2014·Nature Communications·Jake Z JacobsMikel Zaratiegui
Nov 29, 2014·Science·Jennifer A Doudna, Emmanuelle Charpentier
Mar 31, 2015·Science·David W Denning, Michael J Bromley
Jun 4, 2015·Biotechnology and Bioengineering·Takayuki ArazoeShigeru Kuwata
Jul 3, 2015·Journal of Natural Products·Kaoru KinoshitaKunio Takahashi
Jul 8, 2015·Microbial Cell Factories·Carlotta RondaAlex Toftgaard Nielsen
Jul 16, 2015·PloS One·Christina S NødvigUffe H Mortensen
Sep 15, 2015·Fungal Genetics and Biology : FG & B·Mariana SchusterRegine Kahmann
Apr 14, 2016·ACS Synthetic Biology·Carsten PohlYvonne Nygård
Apr 26, 2016·Nature·Emily Waltz
Sep 10, 2016·ACS Synthetic Biology·Jakob WeberAxel A Brakhage
Oct 7, 2016·PloS One·Samantha D M ArrasJames A Fraser
Oct 22, 2016·Scientific Reports·Ludovic EnklerFabrice Jossinet
Mar 14, 2017·Fungal Genetics and Biology : FG & B·Maximilian WenderothReinhard Fischer
Oct 14, 2014·Fungal biology and biotechnology·Alexander Idnurm, Vera Meyer
Jul 15, 2015·Fungal biology and biotechnology·Toru Matsu-UraChristian Hong

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Citations

Aug 21, 2019·Microorganisms·Rahul Mahadev ShelakeJae-Yean Kim
May 28, 2019·Frontiers in Plant Science·Muntazir MushtaqRomesh Kumar Salgotra
Jun 9, 2020·Frontiers in Microbiology·Shweta Jaiswal, Pratyoosh Shukla
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín

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Methods Mentioned

BETA
genetic modifications

Software Mentioned

EasyClone
- Marker Free

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