The critical influence of temperature upon trifluorothymidine resistance in mouse lymphoma L5178Y/TK 3.7.2C cells.

Mutation Research
D E Amacher, G N Turner

Abstract

L5178Y/TK 3.7.2C cells are used for the assessment of chemical mutagenesis caused by presumptive TK gene mutations or multiple loci mutations affecting the TK locus that result in dose-related increases in resistance to the toxic thymidine analog, trifluorothymidine (TFT). This study was based upon our general observation that the incidence of TFTres in these cells could vary with the incubation temperature. As a result of these studies, we found that: (1) a substantial proportion of presumptive TK-/- variants produced by the mutagens 2-aminofluorene (2-AF), N-acetylaminofluorene (AAF), benzo[a]pyrene (B[a]P), 3-methylcholanthrene (3MCA), hycanthone methanesulfonate (Hyc), or methyl methanesulfonate (MMS) are more resistant to TFT at 37 degrees C than at 28 degrees C (or 39 degrees C than at 33 degrees C), (2) the loss of resistance to TFT was most notable in the small-colony variant population, (3) mutagen-derived variants become less resistant as the TFT concentration is increased from 4 micrograms/ml to 50 micrograms/ml, an effect that is more pronounced at 28 degrees C than at 37 degrees C, and (4) stock 3.7.2C cells develop a persistent TFTres due to sharply decreased TK activity when exposed to 40 degrees C for at least 2...Continue Reading

References

Jul 1, 1976·Experimental Cell Research·T Shiomi, K Sato
Jan 1, 1985·Environmental Mutagenesis·D E Amacher, E M Dunn
Nov 1, 1985·Toxicology and Industrial Health·K A Palmer, R De Giovanni-Donnelly
Feb 21, 1966·Biochimica Et Biophysica Acta·J W Littlefield
Oct 1, 1974·Experimental Cell Research·K Sato, T Shiomi
Dec 16, 1974·Biochimica Et Biophysica Acta·P G Plagemann, D P Richey
Jan 1, 1983·Annals of the New York Academy of Sciences·D E Amacher, G N Turner
Aug 20, 1962·Biochimica Et Biophysica Acta·J A JACQUEZ

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