The critical role of human transcriptional repressor CTCF mRNA up-regulation in the induction of anti-HIV-1 responses in CD4(+) T cells.

Immunology Letters
Yuchang LiMalgorzata Simm

Abstract

We have employed our CD4(+) T cell model named HIV-1 resistance factor (HRF(+)) to study the inducible anti-HIV-1 responses mediated through novel soluble molecules. We found that exposure to the soluble products of HRF(+) cells activated CCCTC-binding factor (CTCF) mRNA expression in HIV-1 susceptible primary and transformed CD4(+) T cells and overlapped with their acquisition of transient resistance to virus. Conversely, the interference with the expression of CTCF gene in HRF(+) cells reversed the resistant phenotype and eliminated the biological potential of their cell culture supernatant to induce "HRF-like" activity in target cells. Band-shift analysis upon the nuclear fractions from HIV-1 resistant cells showed that CTCF protein bound to HIV-1 promoter and this binding prevented the formation of NF-kappaB/LTR complex. This evidence suggests that CTCF is an intracellular effector of HRF activity and that the acquisition of resistance to HIV-1 in CD4(+) T cells is a consequence of the prior activation of CTCF gene by the soluble entity secreted by HRF(+) cells.

References

Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L WilleyM A Martin
Mar 1, 1994·AIDS Research and Human Retroviruses·E Aguilar-CordovaJ W Belmont
Aug 30, 2001·Trends in Genetics : TIG·R OhlssonV Lobanenkov
Aug 23, 2002·Seminars in Cancer Biology·Elena M KlenovaVictor V Lobanenkov
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Katherine L Dunn, James R Davie
May 12, 2004·Immunology Letters·Alex KartvelishviliMalgorzata Simm
Jul 2, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elena KlenovaJohn P Quinn
Aug 12, 2004·FEBS Letters·Lars DrueppelChristian Berens
May 30, 2006·Journal of Virology·Charles M ChauPaul M Lieberman
Jul 20, 2007·Immunology and Cell Biology·Guanhua LiMalgorzata Simm

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