Abstract
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm. Tyrosine kinase inhibitors (TKIs) are commonly used to treat CML; however, drug resistance of CML cells to TKIs has limited their clinical application. Shikonin, a traditional Chinese herb, has long been used to treat leukemia in China, but the roles and related molecular mechanisms of shikonin treatment in CML remain unclear. Here, we aimed to evaluate the effects of shikonin on the proliferation, apoptosis, and migration of K562 cells, a CML cell line. Firstly, K562 cell proliferation and apoptosis were tested by CCK8 assay and flow cytometry with Annexin V-FITC/PI staining. Cell migration was measured by Transwell migration assay. In addition, western blot was performed to determine the proteins (PI3K, Bax, Bcl-2, cleaved caspase-3, PTEN, p-AKT, AKT, CXCR4, SDF-1, CD44) involved in the mechanism of action of shikonin. Finally, neutrophils from peripheral blood of CML patients were obtained, and cell proliferation and apoptosis were tested by CCK8 assay and flow cytometry. Shikonin reduced the proliferation of K562 cells in a time- and dose-dependent manner and promoted the apoptosis of K562 cells. Moreover, shikonin increased the PTEN level and inactivated the ...Continue Reading
Citations
Jun 7, 2019·Journal of Cellular Physiology·Chaosen YueJixiang Wu
Oct 17, 2019·Cell Biology International·Xiaojing Li, Xianpeng Zeng
Feb 29, 2020·Journal of Hepatocellular Carcinoma·Tong LiuChuanyong Guo
Mar 24, 2020·Journal of Enzyme Inhibition and Medicinal Chemistry·Xiao-Bao ShenXinhua Liu
Mar 11, 2020·Nanotechnology·Daijia ShenYanli Guo
Mar 28, 2020·Clinical and Experimental Pharmacology & Physiology·Jian LiHongwei Liu
Jan 10, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Meng-Yao SunQing-Feng Tang
Sep 26, 2020·Cell Death & Disease·Rui LiuYuanyan Liu
Jun 26, 2020·Frontiers in Pharmacology·Fangfang WangJinshan Tang
Nov 14, 2019·Chinese Medicine·Hua LuoYitao Wang
Sep 26, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chuanjie GuoCheng Peng
Nov 5, 2019·Chemico-biological Interactions·Hyllana C D MedeirosTiago Rodrigues
Feb 3, 2021·Food & Function·Zhan-Long LiLin-Wu Ran
Oct 23, 2020·International Journal of Molecular Sciences·Wenrui LiLi Sun
Oct 29, 2020·BMC Complementary Medicine and Therapies·Biting WangYun Tang
Apr 4, 2021·Nutrients·Zeljko TodorovicNebojsa Arsenijevic
Apr 11, 2021·Bioorganic Chemistry·Yingying MaYonghua Yang
Jun 3, 2021·Cancers·Christian Boni, Claudio Sorio
Aug 28, 2021·Cancers·Federica RascioElena Ranieri