The crk3 gene of Leishmania mexicana encodes a stage-regulated cdc2-related histone H1 kinase that associates with p12.

The Journal of Biological Chemistry
Karen M GrantJ C Mottram

Abstract

A cdc2-related protein kinase gene, crk3, has been isolated from the parasitic protozoan Leishmania mexicana. Data presented here suggests that crk3 is a good candidate to be the leishmanial cdc2 homologue but that the parasite protein has some characteristics which distinguish it from mammalian cdc2. crk3 is predicted to encode a 35.6-kDa protein with 54% sequence identity with the human cyclin-dependent kinase cdc2 and 78% identity with the Trypanosoma brucei CRK3. The trypanosomatid CRK3 proteins have an unusual, poorly conserved 19-amino acid N-terminal extension not present in human cdc2. crk3 is single copy, and there is 5-fold higher mRNA in the replicative promastigote life-cycle stage than in the non-dividing metacyclic form or mammalian amastigote form. A leishmanial suc-binding cdc2-related kinase (SBCRK) histone H1 kinase, has previously been described which binds the yeast protein, p13(suc1), and that has stage-regulated activity (Mottram J. C., Kinnaird, J., Shiels, B. R., Tait, A., and Barry, J. D. (1993) J. Biol. Chem. 268, 21044-21051). CRK3 from cell extracts of the three life-cycle stages was found to bind p13(suc1) and the leishmanial homologue p12(cks1). CRK3 fused with six histidines at the C terminus was ...Continue Reading

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Citations

Apr 29, 2011·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Bhavna Chawla, Rentala Madhubala
Mar 17, 2004·Biochimica Et Biophysica Acta·Christian Doerig
Nov 23, 2000·Molecular and Biochemical Parasitology·T C HammartonJ C Mottram
Feb 13, 2001·Molecular and Biochemical Parasitology·J J Van Hellemond, J C Mottram
Apr 11, 2001·Molecular and Biochemical Parasitology·P HassanJ C Mottram
Jan 30, 2002·Molecular and Biochemical Parasitology·Rafael Marques PortoSergio Schenkman
May 30, 2002·Molecular and Biochemical Parasitology·María I SantoriMaría T Téllez-Iñón
Nov 11, 1999·Parasitology Today·P G McKeanD F Smith
Feb 1, 2000·Parasitology Today·M Parsons, L Ruben
Oct 16, 2002·Trends in Parasitology·Christian DoerigJeremy C Mottram
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Karen M GrantJeremy C Mottram
Apr 13, 2011·PLoS Neglected Tropical Diseases·Roderick G WalkerJeremy C Mottram
Feb 25, 2014·Journal of Cancer Therapy·Vinayak Khattar, Jaideep V Thottassery
May 3, 2018·Expert Opinion on Therapeutic Targets·Shyam Sundar, Bhawana Singh
Apr 20, 2002·The Journal of Biological Chemistry·Marie KnockaertLaurent Meijer
Feb 8, 2005·Molecular and Biochemical Parasitology·Julia Pinheiro Chagas da CunhaSergio Schenkman
Oct 4, 2005·Biochimica Et Biophysica Acta·Christina NaulaJeremy C Mottram
Sep 26, 2014·Chemical Reviews·Hidayat HussainSimon Gibbons
Feb 1, 2018·ACS Infectious Diseases·Nathaniel G JonesJeremy C Mottram
Oct 8, 2015·Chemical Reviews·Christopher MerrittMichael P Pollastri

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