The crystal structure of Helicobacter cysteine-rich protein C at 2.0 A resolution: similar peptide-binding sites in TPR and SEL1-like repeat proteins

Journal of Molecular Biology
L LüthyPeer R E Mittl

Abstract

Helicobacter pylori is a Gram-negative human pathogen that infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. Bacterial cell-surface and secreted proteins often play an important role in pathogen-host interactions and are thought to be selective mediators for the pathology of the infection. The Helicobacter cysteine-rich proteins (Hcp) represent a large family of secreted proteins that seem to be specific for microorganisms from the epsilon-subfamily of proteobacteria. Although significantly elevated levels of anti-Hcp antibodies were observed in many patients infected with H.pylori, details on the biological functions of Hcp proteins are sparse. Hcps belong to a large family of Sel1-like multi-repeat proteins. The crystal structure of HcpC was refined at 2.0 A resolution and revealed a super-helical topology composed of seven disulfide bridged alpha/alpha-repeats, an N-terminal capping helix and an extended C-terminal coil consisting of alternating hydrophobic and hydrophilic residues. In the crystal packing, the C-terminal coil interacts with the concave surface of a symmetry-related HcpC super-helix. A hydrophobic pocket and a cluster of negatively charged residues reco...Continue Reading

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·E PapiniC Montecucco
Dec 20, 1993·Journal of Molecular Biology·M C Lawrence, P M Colman
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J K MyersJ M Scholtz
Jun 15, 1995·Structure·G J Kleywegt, T A Jones
Aug 1, 1997·Current Opinion in Immunology·J L TelfordP Chiara
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
May 29, 1998·Infection and Immunity·P CaoT L Cover
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Feb 3, 1999·Annals of the New York Academy of Sciences·M M Walker, J E Crabtree
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Mar 26, 1999·Protein Science : a Publication of the Protein Society·M NayalB Honig
May 21, 1999·Science·A CovacciR Rappuoli
Aug 10, 1999·Journal of Bacteriology·P KrishnamurthyS H Phadnis
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G L Blatch, M Lässle
Dec 22, 1999·JAMA : the Journal of the American Medical Association·M J Blaser
Apr 5, 2000·The Journal of Biological Chemistry·P R MittlM G Grütter
Jun 10, 2000·Microbiology·S L CirilloJ D Cirillo
Dec 2, 2000·Nature Structural Biology·G J GattoJ M Berg
Sep 12, 2001·Journal of Structural Biology·M A AndradeC P Ponting
Dec 26, 2001·Nucleic Acids Research·Ivica LetunicPeer Bork
Jan 29, 2002·Journal of Molecular Biology·Oliviero Carugo, Sándor Pongor
Jun 18, 2002·Infection and Immunity·Dirk BumannPeter R Jungblut
Aug 29, 2002·Helicobacter·Markus GerhardMichael Naumann
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jul 8, 2011·Current Microbiology·Bernd RoschitzkiPeer R E Mittl
Dec 19, 2012·Computational Biology and Chemistry·Steffen LindertJens Meiler
Apr 12, 2016·Scientific Reports·Hanbin JeongChangwook Lee
Dec 7, 2005·Journal of Cellular Physiology·Ida BiunnoSylvie Ménard
Dec 9, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Hanbin JeongChangwook Lee
Feb 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jacob O BrunkardPatricia Zambryski
May 28, 2011·Journal of Cellular Physiology·Monica CattaneoIda Biunno
May 1, 2012·Protein Science : a Publication of the Protein Society·Chaithanya MadhurantakamPeer R E Mittl
Jul 12, 2016·FEBS Letters·Xiao-Yun YangYu-Hui Dong
Aug 23, 2018·Nature Communications·Jiwei LiuHarry H Low
May 2, 2020·Acta Crystallographica. Section F, Structural Biology Communications·Haiying WenYuhui Dong
Mar 20, 2019·EMBO Molecular Medicine·Karthik MohanrajAgnieszka Chacinska
May 21, 2020·Frontiers in Cellular and Infection Microbiology·Jaime Vazquez-Lopez, Fernando Navarro-Garcia
Jul 28, 2006·Cellular Signalling·Peer R E Mittl, Wulf Schneider-Brachert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.