The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase

Biochemistry
D H HarrisonH M Miziorko

Abstract

The essential photosynthetic enzyme phosphoribulokinase (PRK) is responsible for the conversion of ribulose 5-phosphate (Ru5P) to ribulose 1,5-bisphosphate, the substrate for the CO2 fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). We have determined the structure of the octameric bacterial form of PRK to a resolution of 2.5 A. The protein is folded into a seven-member mixed beta-sheet surrounded by alpha-helices, giving the overall appearance of the nucleotide monophosphate family of kinases. Homology with the nucleotide monophosphate kinases suggests a number of amino acid residues that are likely to be important in catalysis and suggests the roles of some amino acid residues that have been mutated prior to the determination of the structure. Further, sequence identity across eukaryotic and prokaryotic species and a calculation of the buried surface area suggests the identity within the octamer of a dimer conserved throughout evolution. The width of the groove leading to the active site is consistent with an oriented molecule of thioredoxin controlling the oxidation state of two cysteines that regulate activity in the eukaryotic enzymes. Although neither Asp 42 nor Asp 169 can be definitively assigned ...Continue Reading

References

Aug 1, 1990·Journal of Protein Chemistry·M A PorterF C Hartman
Jan 20, 1988·Journal of Molecular Biology·D DreusickeG E Schulz
Jul 25, 1988·Nucleic Acids Research·K R Roesler, W L Ogren
Nov 17, 1986·European Journal of Biochemistry·G E SchulzR H Schirmer
Feb 11, 1994·Journal of Molecular Biology·H J Müller-Dieckmann, G E Schulz
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Feb 23, 1996·Journal of Molecular Biology·G J SchlaudererG E Schulz

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Citations

Oct 22, 2003·Journal of Molecular Biology·Detlef D LeipeL Aravind
Sep 11, 2002·Archives of Biochemistry and Biophysics·Jennifer A Runquist, Henry M Miziorko
Apr 2, 2008·Antioxidants & Redox Signaling·Peter Schürmann, Bob B Buchanan
Aug 19, 2007·Plant & Cell Physiology·Christine OesterheltRenate Scheibe
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
Dec 3, 2015·Acta Crystallographica. Section D, Biological Crystallography·Alessandra Del GiudiceFrancesca Sparla
Sep 6, 2005·Journal of Molecular Biology·Paul Maragakis, Martin Karplus
Jan 16, 2007·Structure·Matthew Bashton, Cyrus Chothia
Jan 14, 2017·Nature Communications·Takunari KonoHiroki Ashida
Mar 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Libero GurrieriSimona Fermani
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Erik JensenBrigitte Gontero
Apr 6, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Robert H WilsonAndreas Bracher
Dec 14, 2001·The Journal of Biological Chemistry·Fabrice MoucheNicolas Boisset
Apr 25, 2021·Trends in Plant Science·Libero GurrieriPaolo Trost

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