The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA

Fulvia BonoElena Conti


The exon junction complex (EJC) plays a major role in posttranscriptional regulation of mRNA in metazoa. The EJC is deposited onto mRNA during splicing and is transported to the cytoplasm where it influences translation, surveillance, and localization of the spliced mRNA. The complex is formed by the association of four proteins (eIF4AIII, Barentsz [Btz], Mago, and Y14), mRNA, and ATP. The 2.2 A resolution structure of the EJC reveals how it stably locks onto mRNA. The DEAD-box protein eIF4AIII encloses an ATP molecule and provides the binding sites for six ribonucleotides. Btz wraps around eIF4AIII and stacks against the 5' nucleotide. An intertwined network of interactions anchors Mago-Y14 and Btz at the interface between the two domains of eIF4AIII, effectively stabilizing the ATP bound state. Comparison with the structure of the eIF4AIII-Btz subcomplex that we have also determined reveals that large conformational changes are required upon EJC assembly and disassembly.


Sep 1, 1994·Molecular and Cellular Biology·J ChengL E Maquat
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
May 23, 2002·Cell·Morgan Huse, John Kuriyan
Jul 18, 2002·Current Biology : CB·Josée Dostie, Gideon Dreyfuss
Jul 20, 2002·Current Opinion in Structural Biology·Kathleen B Hall
Apr 22, 2003·Genes & Development·Hang Shi, Rui-Ming Xu
Apr 26, 2003·Trends in Biochemical Sciences·Hervé Le HirMelissa J Moore
Apr 30, 2003·Molecular Cell·Niels H GehringAndreas E Kulozik
May 6, 2003·Nature Structural Biology·Sébastien FribourgElena Conti
Jun 5, 2003·Current Biology : CB·Chi-Kong LauGregory D Van Duyne
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Heather L WiegandBryan R Cullen
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Maria A FerraiuoloNahum Sonenberg
Mar 23, 2004·Nature Structural & Molecular Biology·Toshiharu ShibuyaMelissa J Moore
May 18, 2004·Current Opinion in Cell Biology·Thomas Ø TangeMelissa J Moore
May 29, 2004·The Journal of Biological Chemistry·Sébastien DegotCatherine Tomasetto
Jun 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 20, 2005·Current Opinion in Cell Biology·Fabrice Lejeune, Lynne E Maquat
May 20, 2005·Current Opinion in Cell Biology·Elena Conti, Elisa Izaurralde
Sep 17, 2005·Genes & Development·Monika ObererGerhard Wagner
Sep 20, 2005·Nature Structural & Molecular Biology·Lionel BallutHervé Le Hir
Dec 13, 2005·Gene·Olivier CordinPatrick Linder
Apr 25, 2006·Cell·Toru SengokuShigeyuki Yokoyama
May 16, 2006·Acta Crystallographica. Section D, Biological Crystallography·Sergei RadaevPeter D Sun

❮ Previous
Next ❯


Jul 5, 2008·Nature Protocols·Gerrit LangerAnastassis Perrakis
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Patrick Linder, Eckhard Jankowsky
Oct 18, 2012·Nature Reviews. Molecular Cell Biology·Stephanie Kervestin, Allan Jacobson
Feb 17, 2009·Nature Structural & Molecular Biology·Holger von MoellerElena Conti
Mar 6, 2012·Nature Structural & Molecular Biology·Andrea Giovanni MurachelliElena Conti
Apr 24, 2012·Nature Structural & Molecular Biology·Roberto MeleroOscar Llorca
Sep 11, 2012·Nature Structural & Molecular Biology·Isabelle BarbosaHervé Le Hir
Oct 23, 2012·Nature Structural & Molecular Biology·Jérôme SaulièreHervé Le Hir
Dec 6, 2012·Nature Structural & Molecular Biology·Oliver Mühlemann
Jun 15, 2007·Nature Structural & Molecular Biology·Katharina BüttnerKarl-Peter Hopfner
Dec 11, 2007·Nature Structural & Molecular Biology·Hala ChamiehHervé Le Hir
Jul 11, 2009·Bioscience Reports·Sharon H M LingHaiwei Song
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yingfeng ChenRick Russell
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fei LiuEckhard Jankowsky
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gretel BuchwaldElena Conti
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Feng LiLarry Simpson
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrei AlexandrovJoan A Steitz
Feb 27, 2009·The Journal of Biological Chemistry·Ruairi CollinsHerwig Schüler
Sep 21, 2013·The Journal of Biological Chemistry·Zbigniew PietrasBen F Luisi
Mar 16, 2012·Molecular Biology of the Cell·Elisabeth DaguenetCatherine Tomasetto
Jun 17, 2011·Journal of Experimental Botany·Eduardo F MufarregeGraciela C Curi
Feb 13, 2008·Nucleic Acids Research·Robert LewisJens Michaelis
Nov 11, 2010·Nucleic Acids Research·Manuel HilbertDagmar Klostermeier
Nov 30, 2010·Nucleic Acids Research·Klaus H NielsenGregers R Andersen
Mar 10, 2010·Nucleic Acids Research·Máté GyimesiMihály Kovács
Jun 21, 2011·Nucleic Acids Research·Michael E BudimanDonna M Driscoll
May 11, 2012·Nucleic Acids Research·Iga KornetaJanusz M Bujnicki
Mar 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Markus G RudolphDagmar Klostermeier
Aug 5, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mark Del Campo, Alan M Lambowitz
Mar 3, 2010·Annual Review of Biophysics·Wei Yang
Jun 25, 2008·Annual Review of Biophysics·Anna Marie Pyle
Aug 23, 2008·RNA·Nadja RozovskyMelissa J Moore
Nov 27, 2008·RNA·Klaus H NielsenGregers R Andersen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.