The crystal violet nuclei staining technique leads to anomalous results in monitoring mammalian cell cultures.

Cytotechnology
J M BerryMichael Butler

Abstract

Nuclear counts determined by crystal violet staining from samples of stationary or microcarrier cultures of hybridomas, CHO or Vero cells were consistently and significantly higher than cell concentrations determined by the trypan blue or Coulter counter methods. This difference was attributed to the presence of a significant proportion of binucleated cells, which are assumed to be 35% of the cell population in the stationary phase of Vero cultures. The proportion of such cells during exponential growth was variable. However, continuous sub-culture of these cells induced a degree of synchrony during growth which resulted in a cyclic variation of the difference between the cell and nuclei counting techniques. This data indicates that care should be taken in interpreting cell culture profiles based solely on crystal violet nuclei staining counts.

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Citations

Sep 26, 2015·Toxicon : Official Journal of the International Society on Toxinology·Katherine J ReidFiona M Young
Apr 28, 2007·Biotechnology Progress·David R Knop, Heather Harrell
Jan 20, 2011·Chemical Biology & Drug Design·Andre StanderAnnie Joubert
May 18, 2016·Cytotechnology·Christopher J FarrellVan M Hoang
Jun 11, 2021·Biotechnology and Bioengineering·Osnat RosenEyal Epstein

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