The CSL transcription factor LAG-1 directly represses hlh-6 expression in C. elegans

Developmental Biology
Vikas Ghai, Jeb Gaudet

Abstract

The Caenorhabditis elegans gene hlh-6 is expressed specifically in pharyngeal glands, one of five distinct pharyngeal cell types. Expression of hlh-6 is controlled by a discrete set of cis-regulatory elements, including a negative element called HRL1. Here we demonstrate that HRL1 is a functional binding site for LAG-1, the CSL transcriptional effector of Notch in C. elegans, and that regulation of hlh-6 by LAG-1 is direct. Regulation of hlh-6 by LAG-1 is strictly negative: removal of HRL1 or LAG-1 regulation results in ectopic expression of hlh-6, but does not affect expression in pharyngeal glands. Furthermore, direct regulation of hlh-6 expression does not appear to involve Notch signaling, contrary to the canonical mechanism by which CSL factors regulate target genes. We also identify an additional cis-regulatory element in the hlh-6 promoter that, together with previously identified elements, is sufficient to overcome repression by LAG-1 and activate hlh-6 expression in pharyngeal glands.

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Citations

Jun 27, 2013·WormBook : the Online Review of C. Elegans Biology·Valerie ReinkePeter Okkema
Jun 24, 2010·Current Opinion in Genetics & Development·Jay D KormishJames D McGhee
Jul 14, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Wahyu Hendrati RaharjoJeb Gaudet
Sep 30, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·Marc Pilon
Feb 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jeb Gaudet, James D McGhee
Apr 3, 2014·Developmental Biology·Kalpana RamakrishnanPeter G Okkema
Apr 22, 2009·Cell·Raphael Kopan, Maria Xenia G Ilagan

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