PMID: 9170258Feb 1, 1997Paper

The culture of chicken embryo fibroblast cells on microcarriers to produce infectious bursal disease virus

Applied Biochemistry and Biotechnology
L ZhangJ Yu

Abstract

The cultures of chicken embryo fibroblast (CEF) cells in flasks, spinner bottles, and bioreactors were studied. The growth and metabolism characteristics of CEF cells and the feasibility of the CEF cell culture in bioreactor were investigated. The plating process of the CEF cells on GT-2 microcarriers in spinner bottles was studied, and a plating kinetic model was presented. The culture of CEF cells in 1.5 L CelliGen bioreactor to produce infectious bursal disease virus (IBDV) had met success. Whereas the additive microcarriers were fed during the culture, the cell density was increased 10 times as against seed cells adhering to microcarriers and the virus titer was as high as 7.5. All the aforementioned experimental results have laid the foundation for high density culture of CEF cells and process scale-up.

References

May 1, 1988·Tsitologiia·O S MorenkovE I Lezhenev
Jan 1, 1983·Annals of the New York Academy of Sciences·E M ScattergoodM R Hilleman
Oct 5, 1988·Biotechnology and Bioengineering·M S CroughanD I Wang
Jan 5, 1994·Biotechnology and Bioengineering·K A HawboldtL A Behie

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·X SunP Hua

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