The cuticle proteins: a putative role for deltamethrin resistance in Culex pipiens pallens

Parasitology Research
Fujin FangChangliang Zhu

Abstract

Insecticide resistance has been a major public health challenge. It is impendent to study the mechanism on insecticide resistance. In our previous study, 14 differentially accumulated insect cuticle proteins (ICPs) based on insecticide resistance proteomes and transcriptomes were found in the deltamethrin-resistant (DR) and -susceptible (DS) strains of Culex pipiens pallens. To investigate if these ICPs are associated with deltamethrin resistance, different transcriptional levels of the 14 ICPs were detected in the DS and DR strains from laboratory and field populations by using quantitative real-time polymerase chain reaction (qRT-PCR). The expression levels of the 14 ICPs were also measured after short-term exposure of the DS strain to deltamethrin. The full-length complementary DNA (cDNA) of CpCPLCG5 gene, which encodes one of the 14 ICPs, was cloned from Cx. pipiens pallens. Homology analysis and phylogenetic analysis were carried out with some other insects. Furthermore, small interfering RNA (siRNA) was used to knockdown the expression level of CpCPLCG5 gene for characterizing its contribution to deltamethrin resistance. The results showed that the expression level of CpCPLCG5 gene was higher in DR strain than in DS strai...Continue Reading

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Citations

Jun 18, 2016·Journal of Medical Entomology·Bingqian LiuChangliang Zhu
Oct 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kai MaBo Shen
Nov 1, 2017·Archives of Insect Biochemistry and Physiology·Wenyun YeChangliang Zhu
Jul 18, 2019·Proceedings. Biological Sciences·Vasileia BalabanidouJohn Vontas
Nov 25, 2019·Parasitology Research·Hong-Xia HuChang-Liang Zhu
Jul 26, 2017·PLoS Neglected Tropical Diseases·Gonçalo SeixasCarla Alexandra Sousa
Jan 8, 2020·Journal of Agricultural and Food Chemistry·Huitang QiQing Yang

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