PMID: 9003353Dec 15, 1996Paper

The Cu,Zn superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration. Evidence for altered subunit interaction in all the bacteriocupreins

The Biochemical Journal
A BattistoniG Rotilio

Abstract

Gel-filtration chromatography experiments performed at high protein concentrations demonstrate that the Cu,Zn superoxide dismutase from Escherichia coli is monomeric irrespective of the buffer and of ionic strength. The catalytic activity of the recombinant enzyme is comparable with that of eukaryotic isoenzymes, indicating that the dimeric structure commonly found in Cu,Zn superoxide dismutases is not necessary to ensure efficient catalysis. The analysis of the amino acid sequences suggests that an altered interaction between subunits occurs in all bacterial Cu,Zn superoxide dismutases. The substitution of hydrophobic residues with charged ones at positions located at the dimer interface of all known Cu,Zn superoxide dismutases could be specifically responsible for the monomeric structure of the E. coli enzyme.

Citations

Aug 9, 2011·New Biotechnology·Kimiyasu IsobeSakayu Shimizu
Jun 16, 2010·Journal of Bioscience and Bioengineering·Yasutaka SasakiSakayu Shimizu
Jul 4, 2006·Biochimica Et Biophysica Acta·Victor Martin Bolanos-Garcia, Owen Richard Davies
May 23, 2001·The Journal of Biological Chemistry·F PacelloA Battistoni
Jun 12, 2014·The Journal of Biological Chemistry·Yasuyuki SakuraiYoshiaki Furukawa
Jan 4, 2020·The Journal of Physical Chemistry. B·Paulo R MouroJorge Chahine
Jun 21, 2001·Archives of Biochemistry and Biophysics·P CioniG B Strambini
Aug 24, 2001·International Journal of Biological Macromolecules·M BozziM Paci
Dec 3, 1999·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·P Dolashka-AngelovaW Voelter
Jan 19, 2000·Clinical Biochemistry·J M MatésI Núñez de Castro
Sep 16, 2004·Journal of Virology·M N BeckerR W Moyer
May 23, 2021·Molecular Biology and Evolution·Gareth S A Wright
Aug 31, 2018·Biomacromolecules·Jason D FiedlerM G Finn

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