Abstract
CXCL10 is a chemokine with potent chemotactic activity for immune and non-immune cells expressing its receptor CXCR3. Previous studies have demonstrated that CXCL10 is involved in myocardial infarction. However, the role of CXCL10 in cardiac microvascular endothelial cell (CMEC) regulation and related mechanisms remains unclear. In this study, we investigated the effects of CXCL10 on the CMEC migration and explored its potential molecular mechanism by wound healing, cell proliferation and viability analysis. Furthermore, migration-related signaling pathways, including FAK, Erk, p38 and Smad, were examined by Western blotting. We found that CXCL10 significantly promotes CMEC migration under normal conditions and during hypoxia/ischemia. However, no significant differences in CMEC proliferation and viability were observed with or without CXCL10 treatment. CXCL10-mediated CMEC migration was greatly blocked by treatment with an anti-CXCR3 antibody. Although CXCL10 treatment promoted phosphorylation and activation of the FAK, Erk, and p38 pathways during hypoxia/ischemia, CXCL10-mediated CMEC migration was significantly blocked by p38 and FAK inhibitors, but not by an Erk inhibitor. Furthermore, CXCL10-mediated FAK activation was su...Continue Reading
References
Jul 1, 1995·The Journal of Experimental Medicine·A L AngiolilloG Tosato
Feb 12, 1998·The New England Journal of Medicine·A D Luster
Jul 11, 1998·The Journal of Biological Chemistry·Y WengJ A DeMartino
Jul 18, 2001·Circulation·T ScarabelliD Latchman
Jul 27, 2001·Journal of Cellular Physiology·L W LoD L Wang
Sep 18, 2001·Heart·R D Rakhit, M S Marber
Dec 31, 2002·Physiological Reviews·Dirk L Brutsaert
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Oct 27, 2004·American Journal of Respiratory and Critical Care Medicine·Marie D BurdickRobert M Strieter
Jan 26, 2005·The Journal of Biological Chemistry·Anindita DasRakesh C Kukreja
Oct 14, 2006·The Journal of Biological Chemistry·Anindita DasRakesh C Kukreja
Apr 5, 2007·Nature Protocols·Chun-Chi LiangJun-Lin Guan
Jun 2, 2007·The Journal of Biological Chemistry·Nam Y Lee, Gerard C Blobe
Oct 16, 2008·Current Vascular Pharmacology·Michel R HoenigFrank W Sellke
Dec 25, 2008·Autoimmunity Reviews·Eun Young LeeYeong Wook Song
Oct 3, 2009·Circulation Research·Marcin BujakNikolaos G Frangogiannis
Sep 22, 2010·PloS One·Gabriele S V CampanellaAndrew D Luster
Aug 2, 2011·Cytokine & Growth Factor Reviews·Mingli LiuJonathan K Stiles
Aug 13, 2011·The Journal of Biological Chemistry·Gwang Hyeon EomSang-Beom Seo
Nov 30, 2011·Molecular and Cellular Biology·Naoto YoshizukaPeiqing Sun
Oct 3, 2012·Vascular Pharmacology·Bei TongYue Dai
Mar 19, 2013·Circulation Journal : Official Journal of the Japanese Circulation Society·Richard E Gilbert
Aug 13, 2013·The Journal of Allergy and Clinical Immunology·Hesam MovassaghAbdelilah S Gounni
Aug 22, 2013·Stem Cells and Development·Fanbiao MengGang Li
Aug 31, 2013·Experimental Cell Research·Elizabeth KiwanukaElof Eriksson
Jan 11, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Pleunie van den BorneDominique P V de Kleijn
Jun 1, 2014·Cellular Signalling·Dalila Naci, Fawzi Aoudjit
Dec 3, 2014·Stem Cells and Development·Linda Miller
Mar 20, 2015·The Journal of Pharmacology and Experimental Therapeutics·David C Ellinsworth
Citations
May 26, 2017·Nature Communications·Nafees RahmanPeter W Zandstra
Apr 22, 2017·Clinical and Experimental Pharmacology & Physiology·Jun WangYanzhang Li
Jan 19, 2019·BMC Cardiovascular Disorders·Shui-Ming LiHong-Ling Jia
Sep 22, 2016·Oncotarget·Ruo-Yu ZhangZhong-Yin Zhang
Mar 21, 2020·Signal Transduction and Targeted Therapy·Dan LiJianfeng Zhou
Dec 31, 2020·Biomolecules·Claudia LeeSudarshan Rajagopal
Nov 5, 2020·F1000Research·Morgan K Groover, Jillian M Richmond
Apr 17, 2020·Signal Transduction and Targeted Therapy·Dan LiJianfeng Zhou
Apr 15, 2019·Biochimie·Fabricio Pereira BatistaMaria Luiza Vilela Oliva