PMID: 2094285Dec 1, 1990Paper

The cyanase operon and cyanate metabolism

FEMS Microbiology Reviews
P M AndersonJ A Fuchs

Abstract

Cyanase is an inducible enzyme in E. coli that catalyzes bicarbonate-dependent decomposition of cyanate. It is encoded as part of an operon we have named the cyn operon, which includes three genes in the following order: cynT (cyanate permease), cynS (cyanase), and cynX (protein of unknown function). The direction of transcription is opposite to that of the lac operon, and the 3'-end of the cyn operon overlaps the 3'-end of the lac operon by 98 nucleotides. The gene cynR (regulatory protein) is located upstream from the cyn operon, and its transcription is opposite that of the cyn operon. The genes of the cyn operon and the cynR gene have been cloned, sequenced and over-expressed. Cyanate at concentrations of about 1 mM is toxic to strains of E. coli lacking the cyanase gene, but strains in which the inducible gene for cyanase is present can grow on cyanate as the sole source of nitrogen at concentrations as high as 20 mM. The presence of cyanase itself is not sufficient to overcome cyanate toxicity--the permease must also be present. Strains lacking the cyanase gene, but having a functional permease gene, are extremely sensitive to cyanate. Uptake of cyanate involves the product of the permease gene in an energy-dependent proc...Continue Reading

References

Jan 1, 1989·Applied and Environmental Microbiology·D A Kunz, O Nagappan
Sep 1, 1989·Journal of Bacteriology·Y C Sung, J A Fuchs
Mar 1, 1987·Journal of General Microbiology·M Guilloton, F Karst
Nov 5, 1987·Journal of Molecular Biology·K H KimP M Anderson
Apr 8, 1986·Biochemistry·P M Anderson, R M Little
Sep 1, 1985·Analytical Biochemistry·M Guilloton, F Karst
Nov 1, 1973·Biochemical and Biophysical Research Communications·P M AndersonA Meister
Jul 1, 1965·Canadian Journal of Biochemistry·A Taussig
Jul 11, 1967·Biochimica Et Biophysica Acta·L A Sluyterman
Dec 1, 1982·Journal of Neurochemistry·J E Rothlein, S M Parsons
Nov 1, 1984·Metabolism: Clinical and Experimental·M OimomiS Baba
Jun 24, 1980·Biochemistry·P M Anderson
Aug 1, 1980·Bulletin of Environmental Contamination and Toxicology·J W DauchyM D Piwoni
Dec 1, 1960·Canadian Journal of Microbiology·A TAUSSIG
Nov 18, 1960·Biochimica Et Biophysica Acta·A TAUSSIG
Sep 1, 1964·Biochemistry·C M ALLEN, M E JONES
Jan 1, 1972·Methods in Enzymology·G R Stark

❮ Previous
Next ❯

Citations

Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·UNKNOWN Brazilian National Genome Project Consortium
Nov 9, 2010·Applied and Environmental Microbiology·Nina A Kamennaya, Anton F Post
Apr 22, 2014·Microbial Pathogenesis·Fengyi Tang, Milton H Saier
Sep 18, 2008·Fungal Genetics and Biology : FG & B·Skander Elleuche, Stefanie Pöggeler
Sep 18, 2009·Protein Science : a Publication of the Protein Society·Gwendowlyn S Knapp, James C Hu
Sep 9, 2000·FEMS Microbiology Reviews·K S Smith, J G Ferry
Mar 19, 2008·Journal of Industrial Microbiology & Biotechnology·Eriana Serpa BarretoMariangela Hungria
Oct 17, 2009·Microbiology·Skander Elleuche, Stefanie Pöggeler
Feb 23, 2020·Applied Microbiology and Biotechnology·Lynn Sophie SchwardmannSkander Elleuche
Mar 28, 2017·Environmental Science and Pollution Research International·Rashad Kebeish, Omar Al-Zoubi
Mar 25, 2017·The ISME Journal·Lucas Moitinho-SilvaTorsten Thomas
Nov 26, 2015·Applied Microbiology and Biotechnology·Mathew P Watts, John W Moreau
May 17, 2017·Applied Microbiology and Biotechnology·Mathew Paul WattsJohn William Moreau
Apr 24, 2019·World Journal of Microbiology & Biotechnology·Monica SharmaSubhankar Chatterjee
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Dec 1, 1993·Microbiological Reviews·M Riley
Jun 15, 2017·Journal of Agricultural and Food Chemistry·Caleb R SchlachterMaksymilian Chruszcz
Dec 29, 2017·Environmental Science & Technology·Irina KurashovaAlexey Kamyshny
Dec 9, 2021·International Journal of Systematic and Evolutionary Microbiology·Shih-Yao LinChiu-Chung Young

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.