The cyclic AMP receptor protein, CRP, is required for both virulence and expression of the minimal CRP regulon in Yersinia pestis biovar microtus.

Infection and Immunity
Lingjun ZhanRuifu Yang

Abstract

The cyclic AMP receptor protein (CRP) is a bacterial regulator that controls more than 100 promoters, including those involved in catabolite repression. In the present study, a null deletion of the crp gene was constructed for Yersinia pestis bv. microtus strain 201. Microarray expression analysis disclosed that at least 6% of Y. pestis genes were affected by this mutation. Further reverse transcription-PCR and electrophoretic mobility shift assay analyses disclosed a set of 37 genes or putative operons to be the direct targets of CRP, and thus they constitute the minimal CRP regulon in Y. pestis. Subsequent primer extension and DNase I footprinting assays mapped transcriptional start sites, core promoter elements, and CRP binding sites within the DNA regions upstream of pla and pst, revealing positive and direct control of these two laterally acquired plasmid genes by CRP. The crp disruption affected both in vitro and in vivo growth of the mutant and led to a >15,000-fold loss of virulence after subcutaneous infection but a <40-fold increase in the 50% lethal dose by intravenous inoculation. Therefore, CRP is required for the virulence of Y. pestis and, particularly, is more important for infection by subcutaneous inoculation....Continue Reading

References

Nov 6, 1992·Science·O A SodeindeJ D Goguen
Jun 22, 1992·FEBS Letters·A V KarlyshevV P Zav'yalov
Jun 1, 1990·Southern Medical Journal·D B Cornfield, I H Lichtenstein
Jun 28, 1973·The New England Journal of Medicine·L T GutmanS L Katz
Mar 1, 1997·Journal of Bacteriology·W VollmerV Braun
Nov 5, 1999·Journal of Molecular Biology·S Busby, R H Ebright
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanE Carniel
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 18, 2002·Infection and Immunity·Shane Petersen, Glenn M Young
Jun 26, 2003·Nucleic Acids Research·Jacques van Helden
Oct 24, 2003·Current Opinion in Microbiology·Agustino Martínez-Antonio, Julio Collado-Vides
Oct 19, 2004·Microbes and Infection·Dongsheng ZhouRuifu Yang
Nov 3, 2004·Nucleic Acids Research·Dongling ZhengStephen D Minchin
Dec 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark AchtmanPaul Keim
Apr 9, 2005·Genes and Immunity·J HuggettA Zumla
Aug 3, 2005·FEMS Microbiology Letters·Dongsheng ZhouRuifu Yang
Sep 27, 2005·Microbes and Infection·Dongsheng ZhouRuifu Yang
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Florent SebbaneB Joseph Hinnebusch
Aug 24, 2006·Cellular and Molecular Life Sciences : CMLS·D Zhou, R Yang
Jan 16, 2008·The Journal of Biological Chemistry·Jee Eun RheeLinda J Kenney

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Citations

Nov 15, 2011·Nature Reviews. Microbiology·Kathleen A McDonough, Ana Rodriguez
Nov 26, 2009·Infection and Immunity·Jason L O'LoughlinScott D Kobayashi
Mar 18, 2009·Infection and Immunity·Dongsheng Zhou, Ruifu Yang
Jan 11, 2011·Journal of Bacteriology·Shirley M EspertGeorge P Munson
May 8, 2013·Canadian Journal of Microbiology·Shi QuMingyuan Liu
Jun 2, 2011·Canadian Journal of Microbiology·He GaoRuifu Yang
Sep 16, 2011·Journal of Infection in Developing Countries·Wei SunRoy Curtiss
Jun 15, 2011·Applied Microbiology and Biotechnology·Jason A RosenzweigAshok K Chopra
Oct 21, 2015·Critical Reviews in Microbiology·Marijke PeetermansPeter Verhamme
Jan 8, 2013·International Journal of Food Microbiology·Li WangDongsheng Zhou
May 18, 2011·Microbial Pathogenesis·Leigh A FeuerbacherDavid Kolodrubetz
Mar 15, 2016·Frontiers in Cellular and Infection Microbiology·Shiyun ChenMatthew S Francis
Oct 7, 2015·Frontiers in Microbiology·Luary C Martínez-Chavarría, Viveka Vadyvaloo
Sep 16, 2014·Microbial Pathogenesis·Stephan P WilliasVladimir L Motin
Jan 8, 2015·Frontiers in Microbiology·Fengjun SunDongsheng Zhou
Nov 12, 2014·Frontiers in Cellular and Infection Microbiology·Gottfried Wilharm, Christine Heider
Nov 5, 2014·Frontiers in Cellular and Infection Microbiology·Ann Kathrin Heroven, Petra Dersch
Mar 21, 2018·Journal of Bacteriology·Jeremy T Ritzert, Wyndham W Lathem
Jan 18, 2020·PLoS Pathogens·Christian TwittenhoffFranz Narberhaus
Jun 12, 2013·Expert Review of Vaccines·Xiaoyi WangRuifu Yang

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