PMID: 9557657Apr 29, 1998Paper

The Cys-rich region of hepatitis A virus cellular receptor 1 is required for binding of hepatitis A virus and protective monoclonal antibody 190/4

Journal of Virology
P ThompsonGerardo G Kaplan

Abstract

The hepatitis A virus cellular receptor 1 (HAVcr-1) cDNA codes for a class I integral membrane glycoprotein, termed havcr-1, of unknown natural function which serves as an African green monkey kidney (AGMK) cell receptor for HAV. The extracellular domain of havcr-1 has an N-terminal Cys-rich region that displays homology with sequences of members of the immunoglobulin superfamily, followed by a Thr/Ser/Pro (TSP)-rich region characteristic of mucin-like O-glycosylated proteins. The havcr-1 glycoprotein contains four putative N-glycosylation sites, two in the Cys-rich region and two in the TSP-rich region. To characterize havcr-1 and define region(s) involved in HAV receptor function, we expressed the TSP-rich region in Escherichia coli fused to glutathione S-transferase and generated antibodies (Ab) in rabbits (anti-GST2 Ab). Western blot analysis with anti-GST2 Ab detected 62- and 65-kDa bands in AGMK cells and 59-, 62-, and 65-kDa bands in dog cells transfected with the HAVcr-1 cDNA (cr5 cells) but not in dog cells transfected with the vector alone (DR2 cells). Treatment of AGMK and cr5 cell extracts with peptide-N-glycosidase F resulted in the collapse of the havcr-1-specific bands into a single band of 56 kDa, which indicate...Continue Reading

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Citations

May 17, 2000·The Journal of General Virology·J Schneider-Schaulies
Jul 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sheng XiaoVijay K Kuchroo
Nov 17, 2004·The Journal of Biological Chemistry·David JiménezJosé M Casasnovas
Aug 31, 2007·Autoimmunity·Lawrence P Kane
Mar 24, 2006·Physiological Genomics·Joshua H LarsonHarris A Lewin
Feb 15, 2005·Journal of Virology·Dino A FeigelstockGerardo G Kaplan
Dec 20, 2013·Clinical Liver Disease·Charles Phan, F Blaine Hollinger
Jul 11, 1998·Journal of Virology·D FeigelstockG G Kaplan
Oct 13, 2020·Clinical Liver Disease·Daniel Shouval

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