PMID: 32154Nov 1, 1978Paper

The cytochemical localization of nucleotide pyrophosphatase activity in plant tissues using naphthyl esters of thymidine-5'-phosphate

The Histochemical Journal
H SierakowskaA L Dawson

Abstract

A cytochemical method for the localization of nucleotide pyrophosphatase activity in plants employing naphthol AS-BI thymidine 5'-monophosphate and alpha-naphthyl thymidine 5'-monophosphate as specific substrates is reported. Biochemical evidence for the validity of this method is presented and the synthesis of the naphthol AS-BI ester is described. The application of this cytochemical technique to shoots of Triticum sp. and roots of Vicia faba has shown nucleotide pyrophosphatase to be ubiquitous in its distribution in these organs and to occur in a structurally-bound form in the cytoplasm. The highest activity was detected in developing fibres adjacent to the leaf vascular bundles, in the coleoptile epidermal and hypodermal cells and in the coleoptile and leaf xylem.

References

May 18, 1976·Biochemistry·H ShinshiT Sugimura
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Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K ShimotohnoK I Miura
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Sep 1, 1973·Plant Physiology·J VandepeuteR Alvarez

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