The Cytochrome bd Complex Is Essential for Chromate and Sulfide Resistance and Is Regulated by a GbsR-Type Regulator, CydE, in Alishewanella Sp. WH16-1

Frontiers in Microbiology
Xian XiaGejiao Wang

Abstract

Sulfate-reducing bacteria are a group of microorganisms that use sulfate as an electron acceptor. These bacteria are useful in the bioremediation of heavy metal pollution since they can reduce/precipitate metals. Previously, we identified the Alishewanella strain WH16-1 from soil of a copper and iron mine and determined that it can reduce sulfate and chromate and that it was tolerant to many heavy metals. In this study, we investigated the chromate reduction mechanism of strain WH16-1 through Tn5 transposon mutagenesis. A cytochrome bd (cytbd) Tn5 mutant was generated (Δcytbd), and a detail analysis showed that the following: (1) gene cydE (coding for a GbsR-type regulator) was co-transcribed with the two subunits coding genes of the Cytochrome bd complex (Cytbd), namely, cydA and cydB, based on RT-PCR analysis, and similar gene arrangements were also found in other Alteromonadaceae family strains; (2) the chromate resistance level was dramatically decreased and chromate reduction efficiency also decreased in strain Δcytbd compared to the wild-type and a complemented strain (Δcytbd-C); (3) Cytbd could catalyze the decomposition of H2O2 according to the analyses of H2O2 decomposition ability, cellular H2O2 contents, H2O2 inhibit...Continue Reading

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Citations

Feb 8, 2019·Applied Microbiology and Biotechnology·Shijuan WuGejiao Wang
Nov 9, 2018·Frontiers in Microbiology·Stefanie RonzheimerErhard Bremer
Jun 3, 2021·Antioxidants·Vitaliy B BorisovElena Forte

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Methods Mentioned

BETA
PCR
Assay
atomic absorption spectrometry
One-Hybrid
electrophoresis
footprinting

Software Mentioned

ClustalW
BLAST
Espript
BLASTP
MEGA

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