The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP

BMC Immunology
Noriyuki TakahashiHirokazu Murakami

Abstract

T-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing. The Th1/Th2 ratio was detected in peripheral blood mononuclear cells via flow cytometry. cITP patients had a higher frequency of the IL-4Rα 576 non-QQ genotype compared to healthy subjects (P = 0.04). cITP patients with the IFN-γ +874 non-AA genotype (high expression type) showed more severe thrombocytopenia than those with the AA genotype (P < 0.05). cITP patients had a significantly higher Th1/Th2 ratio than control patients (P < 0.01); this ratio was inversely correlated with platelet counts. Furthermore, patients with both IFN-γ +874 non-AA genotype (high expression type) and IFN-γR -611 non-AA genotype (high-function type) had a significantly higher Th1/Th2 ratio (P < 0.05). The cytokine polymorphisms affecting Th1/...Continue Reading

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Citations

Mar 19, 2020·Chinese Medical Journal·Xiao-Mei ZhangZong-Hong Shao
Mar 23, 2020·Cellular Immunology·Milos KosticGoran Marjanović
Mar 30, 2021·International Journal of General Medicine·Xiuxiu LinChangcheng Zheng
May 4, 2021·Experimental Biology and Medicine·Qinzhi LiXinhong Guo
Jul 25, 2021·International Journal of Molecular Sciences·Iason ThomasAthanasia Mouzaki
Jul 31, 2021·Journal of Clinical Laboratory Analysis·Ting WangZonghong Shao

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Methods Mentioned

BETA
electrophoresis
PCR
flow cytometry
FACS
flow

Software Mentioned

FACS Diva

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