The cytomegalovirus US28 protein binds multiple CC chemokines with high affinity

Biochemical and Biophysical Research Communications
D E KuhnP E Kolattukudy

Abstract

Human cytomegalovirus encodes several proteins with high similarity to seven transmembrane domain receptors. We investigated the ability of one of these proteins, the product of the US28 open reading frame, to bind various chemoattractant ligands. When transfected into COS-7 cells, the US28 product conferred high affinity binding to the labeled chemokines monocyte chemoattractant protein-1 (MCP-1) (Kd = 6.0 x 10(-10) M) and RANTES (Kd = 2.7 x 10(-10) M). Binding of these labeled ligands could be competed by the unlabeled macrophage inflammatory proteins MIP-1 alpha and MIP-1 beta, with Kd values in the range 1.2 x 10(-9) to 7.5 x 10(-9) M. Comparisons of the sequences of US28 and other receptors that bind chemokines should help to define regions responsible for receptor-ligand interactions.

Citations

Nov 4, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E ParadowskaZ J Leśnikowski
Nov 26, 1999·Immunology and Cell Biology·S Mahalingam, G Karupiah
Sep 13, 2002·Immunology and Cell Biology·Shaun R McColl
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Khalid S A Khabar, Stephen J Polyak
Oct 5, 2001·Clinical Microbiology Reviews·S W Chensue
Jan 5, 2002·Journal of Virology·Yvonne K GruijthuijsenCornelis Vink
Jul 29, 1999·American Journal of Respiratory Cell and Molecular Biology·G W HunninghakeL J Geist
Oct 28, 2010·The Journal of Clinical Investigation·Gerold BongersSergio A Lira
Jan 13, 2009·Virology Journal·Mindy Miller-Kittrell, Tim E Sparer
Jan 1, 2010·Herpesviridae·Jennifer VomaskeDaniel N Streblow
Jun 5, 2010·Future Microbiology·Barry SlobedmanAllison Abendroth
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·L Rasmussen
Oct 25, 2012·Annual Review of Pharmacology and Toxicology·Silvia MontanerJ Silvio Gutkind
Mar 10, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S ShanmughapriyaK Natarajaseenivasan
Mar 26, 2003·Microbes and Infection·Peter J Holst, Mette M Rosenkilde
Jul 17, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Amita YadavSarika Arora
Sep 11, 2008·Protein Expression and Purification·Abhiram DukkipatiK Christopher Garcia
Dec 7, 2007·Life Sciences·Joseph D Sherrill, William E Miller
Aug 16, 2005·The FEBS Journal·Olivier PleskoffJean Claude Ameisen
Oct 22, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R B Freeman
Feb 1, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C Smith, R Khanna
Feb 15, 2012·Cellular Microbiology·Felicia GoodrumPatricia Zagallo
Jun 3, 2004·Human Immunology·Susan Michelson
Jan 13, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Koen W R van CleefCornelis Vink
Jul 6, 2005·Cytokine & Growth Factor Reviews·Massimo LocatiAlberto Mantovani
Jul 7, 2005·Cytokine & Growth Factor Reviews·Nicole F NeelAnn Richmond
Dec 20, 2007·The Journal of General Virology·Melissa P M Stropes, William E Miller
Jun 21, 2011·Biochemical Pharmacology·Pia TschischeMaria Waldhoer
Mar 26, 2015·Frontiers in Pharmacology·Sabrina M de MunnikHenry F Vischer
May 20, 1999·Experimental and Molecular Pathology·W J Martin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.