PMID: 7538138May 1, 1995Paper

The cytoplasmic domain of L-selectin interacts with cytoskeletal proteins via alpha-actinin: receptor positioning in microvilli does not require interaction with alpha-actinin

The Journal of Cell Biology
F M PavalkoGeoffrey S Kansas

Abstract

The leukocyte adhesion molecule L-selectin mediates binding to lymph node high endothelial venules (HEV) and contributes to leukocyte rolling on endothelium at sites of inflammation. Previously, it was shown that truncation of the L-selectin cytoplasmic tail by 11 amino acids abolished binding to lymph node HEV and leukocyte rolling in vivo, but the molecular basis for that observation was not determined. This study examined potential interactions between L-selectin and cytoskeletal proteins. We found that the cytoplasmic domain of L-selectin interacts directly with the cytoplasmic actin-binding protein alpha-actinin and forms a complex with vinculin and possibly talin. Solid phase binding assays using the full-length L-selectin cytoplasmic domain bound to microtiter wells demonstrated direct, specific, and saturable binding of purified alpha-actinin to L-selectin (Kd = 550 nM), but no direct binding of purified talin or vinculin. Interestingly, talin potentiated binding of alpha-actinin to the L-selectin cytoplasmic domain peptide despite the fact that direct binding of talin to L-selectin could not be measured. Vinculin binding to the L-selectin cytoplasmic domain peptide was detectable only in the presence of alpha-actinin. ...Continue Reading

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Oct 1, 1992·European Journal of Immunology·E J von AsmuthW A Buurman
Aug 1, 1990·The Journal of Cell Biology·C A OteyK Burridge
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jul 31, 1987·Biochemical and Biophysical Research Communications·D H WachsstockS Lin
Apr 19, 1984·Nature·K Burridge, P Mangeat
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S L ErlandsenR D Nelson
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson

❮ Previous
Next ❯

Citations

May 1, 1996·Journal of Cellular Physiology·D K HsuJ A Winkles
Jul 27, 2005·Cancer Immunology, Immunotherapy : CII·Qing ChenSharon S Evans
Sep 18, 2009·Immunologic Research·Daniel T FisherSharon S Evans
Sep 27, 1996·Journal of Immunological Methods·A PierresP Bongrand
Jun 30, 1998·Prostaglandins & Other Lipid Mediators·S D CatzN B Sterin-Speziale
Sep 17, 2002·Current Opinion in Cell Biology·Rodger P McEver
Oct 1, 1996·Current Opinion in Cell Biology·D DunonB A Imhof
Feb 1, 1997·Current Opinion in Cell Biology·K M Yamada, B Geiger
Oct 1, 1996·Current Opinion in Structural Biology·P R Crocker, T Feizi
Mar 26, 2004·Nature Reviews. Immunology·Miguel Vicente-Manzanares, Francisco Sánchez-Madrid
Jul 6, 2001·The Biochemical Journal·Y CaoA Zolkiewska
Jan 9, 2009·The Journal of Biological Chemistry·David J KillockAleksandar Ivetic
Oct 9, 1998·The Journal of Experimental Medicine·X LiT F Tedder
Apr 15, 1997·Annals of the New York Academy of Sciences·M Yoshida, M A Gimbrone
May 18, 2007·Annual Review of Biochemistry·Soichiro Yamada, W James Nelson
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michelangelo FotiJean-Louis Carpentier
Apr 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ulrich S Schwarz, Ronen Alon
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Rodger P McEver, Cheng Zhu
Aug 19, 2006·Immunological Investigations·Daniel T FisherSharon S Evans
Sep 3, 2005·Immunological Investigations·Michelle M AppenheimerSharon S Evans
Jan 25, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Sharon S EvansQing Chen
Aug 10, 2000·The Journal of Cell Biology·M M Parast, C A Otey
Jun 15, 1999·The Journal of Cell Biology·B Wójciak-StothardA J Ridley
May 19, 2004·FEBS Letters·Mikko RöntyOlli Carpén
Mar 10, 1997·The Journal of Cell Biology·B KreftR Gessner
Mar 26, 2002·FEBS Letters·Kristina Djinovic-CarugoPaul Young
Oct 28, 1998·Cell Adhesion and Communication·A PierresP Bongrand
Jan 10, 2013·The International Journal of Biochemistry & Cell Biology·Aleksandar Ivetic
May 7, 2011·European Journal of Cell Biology·Stefanie WedepohlRudolf Tauber
Mar 30, 2010·Biochemical and Biophysical Research Communications·Michael G AndrzejewskiAndreas Ludwig
Oct 5, 2007·Journal of Immunological Methods·Dooyoung Lee, Michael R King
Apr 15, 2004·Cell Motility and the Cytoskeleton·Carol A Otey, Olli Carpen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.