The cytoplasmic tail of the severe acute respiratory syndrome coronavirus spike protein contains a novel endoplasmic reticulum retrieval signal that binds COPI and promotes interaction with membrane protein

Journal of Virology
Corrin E McBrideCarolyn E Machamer

Abstract

Like other coronaviruses, severe acute respiratory syndrome coronavirus (SARS CoV) assembles at and buds into the lumen of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC). Accumulation of the viral envelope proteins at this compartment is a prerequisite for virus assembly. Previously, we reported the identification of a dibasic motif (KxHxx) in the cytoplasmic tail of the SARS CoV spike (S) protein that was similar to a canonical dilysine ER retrieval signal. Here we demonstrate that this motif is a novel and functional ER retrieval signal which reduced the rate of traffic of the full-length S protein through the Golgi complex. The KxHxx motif also partially retained two different reporter proteins in the ERGIC region and reduced their rates of trafficking, although the motif was less potent than the canonical dilysine signal. The dibasic motif bound the coatomer complex I (COPI) in an in vitro binding assay, suggesting that ER retrieval may contribute to the accumulation of SARS CoV S protein near the virus assembly site for interaction with other viral structural proteins. In support of this, we found that the dibasic motif on the SARS S protein was required for its localization to the ERGIC/Golgi region...Continue Reading

References

Oct 1, 1992·Seminars in Cell Biology·G Griffiths, P Rottier
Oct 1, 1991·The Journal of Cell Biology·A M Swift, C E Machamer
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W J LeonardW C Greene
Oct 1, 1995·The Journal of Cell Biology·D J OpsteltenP J Rottier
Sep 1, 1993·The Journal of Cell Biology·O A WeiszC E Machamer
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A GottliebB M Babior
Jan 1, 1996·Annual Review of Cell and Developmental Biology·R D Teasdale, M R Jackson
Nov 26, 1997·Journal of Virology·V P Nguyen, B G Hogue
May 4, 2000·American Journal of Physiology. Cell Physiology·D MaouyoM H Montrose
Jun 24, 2000·The Journal of Biological Chemistry·M GomezF Perez
Feb 13, 2001·Virology·T M Gallagher, M J Buchmeier
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·N ZerangueB Schwappach
Mar 30, 2001·The Journal of General Physiology·M Grabe, G Oster
Jan 5, 2002·Journal of Virology·Emily Corse, Carolyn E Machamer
Feb 28, 2002·Biochemical and Biophysical Research Communications·Birgit Hardt, Ernst Bause
Jul 20, 2002·The Journal of Biological Chemistry·Stuart W Hicks, Carolyn E Machamer
Mar 29, 2003·Journal of Virology·Lili Kuo, Paul S Masters
May 6, 2003·Science·Marco A MarraRachel L Roper
Jun 5, 2003·Lancet·John M NichollsJ S Malik Peiris
Oct 28, 2003·Archives of Virology·J M GonzálezLuis Enjuanes
Dec 3, 2003·The Journal of General Virology·M-L NgA-E Ling
Mar 5, 2004·The Journal of Biological Chemistry·Brian TripetRobert S Hodges
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Mar 20, 2004·Emerging Infectious Diseases·Cynthia S GoldsmithSherif R Zaki
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himani BishtBernard Moss
May 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Berend Jan BoschPeter J M Rottier

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Citations

Apr 10, 2015·Viruses·Makoto Ujike, Fumihiro Taguchi
Mar 10, 2015·Virology·Christian Harak, Volker Lohmann
Mar 31, 2015·Frontiers in Biology·Rebecca M Fox, Deborah J Andrew
Mar 21, 2018·The Journal of General Virology·Jeanne M SiskCarolyn E Machamer
Oct 20, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying WangLi Liu
Jul 15, 2020·The Journal of Biological Chemistry·Ella HartenianBritt A Glaunsinger
Jan 1, 2016·Toxicology and Environmental Health Sciences·Simranjeet Singh SekhonYang-Hoon Kim
Jul 28, 2020·Cellular and Molecular Life Sciences : CMLS·Rajanish GiriVladimir N Uversky
Aug 6, 2020·Biological Procedures Online·Reza Zolfaghari EmamehMajid Khoshmirsafa
Jul 30, 2020·The Journal of Cell Biology·Daria SicariEric Chevet
Oct 30, 2020·Biochemical Society Transactions·Yu-Jie ChenBilly Tsai
Jan 8, 2021·Cell·Wilfredo F Garcia-BeltranAlejandro B Balazs

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