The Cytoprotective Effects of Human Endothelial Progenitor Cell-Conditioned Medium Against an Ischemic Insult Are Not Dependent on VEGF and IL-8

Cell Transplantation
Stefano Di SantoHans Rudolf Widmer

Abstract

Endothelial progenitor cells (EPCs) promote revascularization and tissue repair mainly by paracrine actions. In the present study, we investigated whether EPC-secreted factors in the form of conditioned medium (EPC-CM) can protect cultured brain microvascular endothelial cells against an ischemic insult. Furthermore, we addressed the type of factors that are involved in the EPC-CM-mediated functions. For that purpose, rat brain-derived endothelial cells (rBCEC4 cell line) were exposed to EPC-CM pretreated with proteolytic digestion, heat inactivation, and lipid extraction. Moreover, the involvement of VEGF and IL-8, as canonical angiogenic factors, was investigated by means of neutralizing antibodies. We demonstrated that EPC-CM significantly protected the rBCEC4 cells against an ischemic insult mimicked by induced oxygen-glucose deprivation followed by reoxygenation. The cytoprotective effect was displayed by higher viable cell numbers and reduced caspase 3/7 activity. Heat inactivation, proteolytic digestion, and lipid extraction resulted in a significantly reduced EPC-CM-dependent increase in rBCEC4 viability, tube formation, and survival following the ischemic challenge. Notably, VEGF and IL-8 neutralization did not affect ...Continue Reading

References

Mar 22, 2005·Experimental Physiology·Paolo Madeddu
Apr 5, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Tongrong HeZvonimir S Katusic
Jul 7, 2005·Nature Reviews. Immunology·Hugh Rosen, Edward J Goetzl
Oct 4, 2005·Journal of Molecular and Cellular Cardiology·Carmen UrbichStefanie Dimmeler
Oct 29, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jiabin GuoSue P Duckles
Aug 4, 2010·Neuroscience Letters·Norihiko NakanoChizuka Ide
Dec 15, 2010·Journal of Molecular and Cellular Cardiology·Carmen UrbichManuel Mayr
May 28, 2011·Brain : a Journal of Neurology·Robert H AndresGary K Steinberg
Nov 23, 2011·Journal of Cellular Physiology·Andria N SmithAnne M Hocking
Mar 6, 2012·Cell Stem Cell·Sudhir H RanganathJeffrey M Karp
Nov 28, 2012·Basic Research in Cardiology·Isabella KanzlerElisa A Liehn
Feb 5, 2013·Biochimica Et Biophysica Acta·Paromita Mukherjee, Sridhar Mani
Feb 5, 2013·Biochimica Et Biophysica Acta·Manousos MakridakisAntonia Vlahou
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jun 22, 2013·Transplantation Research·Diana F Anthony, Paul G Shiels
Jul 6, 2013·Biochimie·Denise DragoStefano Pluchino
Jul 9, 2013·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Weiwei LinXiaodong Wang
Dec 5, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Katya J ParkAndrew J Baker
Jan 10, 2014·The Journal of Pharmacology and Experimental Therapeutics·Li ChenAustin M Guo
Mar 7, 2014·Pharmacology & Therapeutics·Annelies BronckaersIvo Lambrichts
Mar 22, 2014·Stem Cells and Development·Lieke C J van den BerkRuurd Torensma
Mar 29, 2014·American Journal of Respiratory Cell and Molecular Biology·Yun Sil ChangWon Soon Park

❮ Previous
Next ❯

Citations

May 24, 2016·Neural Regeneration Research·Stefano Di Santo, Hans Rudolf Widmer
Dec 8, 2017·Regenerative Medicine·Khawaja Husnain HaiderMateq Ali Al-Reshidi

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection

Software Mentioned

Prism
ImageJ
GraphPad

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.