The Cytosolic pH of Individual Saccharomyces cerevisiae Cells Is a Key Factor in Acetic Acid Tolerance

Applied and Environmental Microbiology
Miguel Fernández-NiñoJoaquín Ariño

Abstract

It was shown recently that individual cells of an isogenic Saccharomyces cerevisiae population show variability in acetic acid tolerance, and this variability affects the quantitative manifestation of the trait at the population level. In the current study, we investigated whether cell-to-cell variability in acetic acid tolerance could be explained by the observed differences in the cytosolic pHs of individual cells immediately before exposure to the acid. Results obtained with cells of the strain CEN.PK113-7D in synthetic medium containing 96 mM acetic acid (pH 4.5) showed a direct correlation between the initial cytosolic pH and the cytosolic pH drop after exposure to the acid. Moreover, only cells with a low initial cytosolic pH, which experienced a less severe drop in cytosolic pH, were able to proliferate. A similar correlation between initial cytosolic pH and cytosolic pH drop was also observed in the more acid-tolerant strain MUCL 11987-9. Interestingly, a fraction of cells in the MUCL 11987-9 population showed initial cytosolic pH values below the minimal cytosolic pH detected in cells of the strain CEN.PK113-7D; consequently, these cells experienced less severe drops in cytosolic pH. Although this might explain in part...Continue Reading

References

Feb 1, 1987·Journal of Bacteriology·J PlessetC S McLaughlin
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Jun 1, 1996·Microbiology·Margarida CasalCecilia Leao
Feb 25, 1999·Journal of Applied Microbiology·R J Lambert, M Stratford
Feb 26, 2000·FEMS Microbiology Letters·M E Pampulha, M C Loureiro-Dias
Jun 23, 2001·Journal of Industrial Microbiology & Biotechnology·N V NarendranathW M Ingledew
May 11, 2005·Nature Reviews. Genetics·Mads KaernJames J Collins
Jul 18, 2006·Nature Reviews. Microbiology·Simon V Avery
Aug 4, 2006·Applied and Environmental Microbiology·Minoska ValliDiethard Mattanovich
Oct 20, 2010·Omics : a Journal of Integrative Biology·Nuno P MiraIsabel Sá-Correia
Oct 27, 2010·Microbial Cell Factories·Nuno P MiraIsabel Sá-Correia
Mar 23, 2011·Biochimica Et Biophysica Acta·Rick OrijGertien J Smits
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 25, 2012·Applied and Environmental Microbiology·Azmat UllahGertien J Smits
Mar 7, 2013·Genetics·Martha S Cyert, Caroline C Philpott
Jun 20, 2013·Frontiers in Microbiology·Azmat UllahGertien J Smits
Jul 17, 2013·International Journal of Food Microbiology·Malcolm StratfordDavid B Archer
Sep 17, 2013·Applied and Environmental Microbiology·Stefan ZdraljevicC Gustavo Pesce
Jan 15, 2014·Frontiers in Microbiology·Nuno P Mira, Miguel C Teixeira

❮ Previous
Next ❯

Citations

May 29, 2016·Applied and Environmental Microbiology·Claudia CapusoniConcetta Compagno
Nov 28, 2019·Applied Microbiology and Biotechnology·Ningzi Guan, Long Liu
May 10, 2017·Biotechnology for Biofuels·Venkatachalam NarayananMagnus Carlquist
Nov 22, 2018·Frontiers in Microbiology·Akaraphol WatcharawipasHiroshi Takagi
Jun 20, 2019·International Journal of Molecular Sciences·Xuhua Xia
Jul 31, 2019·International Journal of Food Microbiology·Jan DijksterhuisPaul Bruinenberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.