PMID: 3773505Nov 1, 1986Paper

The cytotoxic effect of surgical glove powder particles on adult human vascular endothelial cell cultures: implications for clinical uses of tissue culture techniques

The Journal of Surgical Research
J B SharefkinN M Rich

Abstract

Clinical use of autogenous endothelial cell (EC) seeding of vascular prostheses (VP) would require reliable methods for EC harvest for immediate seeding or primary culture in a hospital or operating room setting. Observation of glove powder particles (GPP) in failed primary adult human saphenous vein EC (AHSVEC) cultures led us to study the effect of surgical GPP on cultured AHSVEC. Addition of GPP to the culture medium of growing ASHVEC cultures reduced the cell counts in a dose-dependent fashion; the mean concentration of GPP required to produce a greater than 50% decrease in cell number was 1.5 +/- 0.8 (SD) X 10(4) GPP/ml (N = 10 experiments), equivalent to a mean dose of 36 micrograms glove powder per milliliter. The effect was seen within 24 hr of addition of GPP and was not due to interference with EC attachment and spreading or to changes in medium osmolality, pH, glucose, electrolyte, Ca2+, or Mg2+ content. Instead, the effect appeared to be due to a filterable toxin added during the final rubber-vulcanizing stage of glove manufacture, since pure cornstarch particles and epichlorhydrin-treated pure cornstarch did not prevent culture growth, whereas 0.2 micron filtrates of medium incubated with GPP taken directly from gl...Continue Reading

References

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Citations

Feb 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D H RobinsonT B Nielsen
Dec 25, 2008·Journal of Assisted Reproduction and Genetics·Antoine HannounJohnny Awwad
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J S RubinS A Aaronson
Sep 1, 1992·Annals of Vascular Surgery·M WelchM G Walker
Feb 8, 2003·Endothelium : Journal of Endothelial Cell Research·Johann MeinhartPeter Zilla
Oct 1, 1992·Journal of Virology·M N PensieroJ Hay

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