The cytotoxic effects of propolis on breast cancer cells involve PI3K/Akt and ERK1/2 pathways, mitochondrial membrane potential, and reactive oxygen species generation

Inflammopharmacology
Yahima Frión-HerreraJosé Maurício Sforcin

Abstract

Propolis has been extensively used to improve health and prevent inflammatory diseases. Different types of Cuban propolis (red, brown and yellow) have been documented. The purpose of this research was to investigate the cytotoxic effects of Cuban red propolis (CP) on MDA MB-231 cell line, since breast cancer is considered one of the most common causes of mortality among women. Antiproliferative and cytotoxic activity of CP against MDA MB-231 cells were determined by the 3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyl tetrazoliumbromide (MTT) and lactate dehydrogenase (LDH) assays. Apoptosis/necrosis, involvement of PI3K/Akt and ERK1/2 pathways, mitochondrial membrane potential and expression of genes were investigated. CP extract exhibited antiproliferative and cytotoxic effects on MDA MB-231 cells, what may be probably related to PI3K/Akt and ERK1/2 pathways. A decreased expression of apoptosis-related genes (TP53, CASP3, BAX and P21) was seen, whereas the expressions of BCL-2, BCL-XL, NOXA and PUMA were unaffected. CP extract induced mitochondrial dysfunction and LDH release, what indicated cell necrosis associated with reactive oxygen species production and decreased cell migration. Our findings provide a basis for future investi...Continue Reading

References

Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Oct 27, 2004·Apoptosis : an International Journal on Programmed Cell Death·M OsakiH Ito
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·Walter Kolch
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Martin OttBoris Zhivotovsky
Aug 19, 2007·Journal of Agricultural and Food Chemistry·Osmany Cuesta-RubioLuca Rastrelli
Oct 29, 2008·Oncogene·T F Franke
Oct 13, 2011·The Journal of Pharmacy and Pharmacology·Maria Angélica Ehara WatanabeJosé Maurício Sforcin
Jun 2, 2012·Nature Reviews. Drug Discovery·Bedrich L EckhardtRobin L Anderson
Dec 25, 2012·International Journal of Clinical Pharmacology and Therapeutics·Ali Haydar AcikelliDavid Díaz-Carballo
May 9, 2014·Chemistry Central Journal·Vassya BankovaBoryana Trusheva
Jan 27, 2015·Chemico-biological Interactions·Gilberto L Pardo AndreuLuciane C Alberici
Feb 28, 2015·Journal of Dietary Supplements·Seema Patel
Apr 30, 2015·The Journal of Pharmacy and Pharmacology·Bruno J ContiJosé M Sforcin
Jun 25, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Ricardo Silva-CarvalhoCristina Almeida-Aguiar
Jul 25, 2015·The Journal of Pharmacy and Pharmacology·Yahima Frión-HerreraJosé Maurício Sforcin
Oct 31, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Zahra ShahsavariSiamak Salami
Feb 13, 2016·Mini Reviews in Medicinal Chemistry·Ying-Yun GuanChao Fang
Mar 19, 2016·Phytotherapy Research : PTR·José M Sforcin

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Citations

Mar 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·João Henrique Maia AssumpçãoCláudia Aparecida Rainho
Jul 11, 2020·Journal of Cancer Prevention·Hui-Fang ChiuChin-Kun Wang
Jan 19, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mengran XuXin Sun
Aug 28, 2021·Nutrients·Ewa Forma, Magdalena Bryś

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Methods Mentioned

BETA
MDA
PCR

Software Mentioned

Image J
GraphPad Prism
GraphPad

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