PMID: 9547256Jun 20, 1998Paper

The D arm of tRNATyr is necessary and sufficient for import into Leishmania mitochondria in vitro

Nucleic Acids Research
S MahapatraSamit Adhya

Abstract

Transfer RNAs are selectively imported from the cytoplasm into mitochondria of kinetoplastid protozoa such as Leishmania . The specific structural features of tRNA which determine selectivity are largely unknown. Using an in organello system from Leishmania , the import signals on tRNATyrand on a synthetic transcript which binds to the same receptor, were studied by deletion and reconstruction analyses. In both cases, short oligoribonucleotides (minihelices) containing the sequence UGGYAGAG were imported with high efficiency in the presence of ATP. This motif is present in the D arm of tRNATyr, as well as in the majority of imported Leishmania tRNAs. Deletion of the D arm, or a point mutation in the conserved motif, reduces importability. The import signal coincides with the binding site for the mitochondrial receptor TAB. tRNAGln, which is not imported, forms non-productive, TAB-independent complexes with the mitochondrial surface. However, the observation that the imported:bound ratio of the D arm minihelix is higher than that of the entire molecule suggests that the post-binding translocation step is constrained in terms of size or structural flexibility. Kinetic studies of minihelix import indicate stepwise insertion of the...Continue Reading

References

Aug 25, 1994·Nucleic Acids Research·S MahapatraS Adhya
Apr 1, 1994·Molecular and Cellular Biology·A SchneiderN Agabian
Apr 1, 1993·Molecular and Biochemical Parasitology·L F LyeY Suyama
Sep 25, 1996·Molecular & General Genetics : MGG·R KumarI Small
Aug 22, 1997·The Journal of Biological Chemistry·S AdhyaS Mahapatra

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Citations

Dec 17, 2008·Current Genetics·Anne-Marie DuchêneLaurence Maréchal-Drouard
Oct 12, 2001·International Journal for Parasitology·A Schneider
May 30, 2001·Molecular and Biochemical Parasitology·R SalavatiK D Stuart
May 30, 2001·Advanced Drug Delivery Reviews·N S EntelisI A Tarassov
Dec 21, 2000·Trends in Cell Biology·A Schneider, L Maréchal-Drouard
Jan 24, 2012·The Journal of Biological Chemistry·David SeidmanStephen Hajduk
Aug 6, 1999·Molecular Biology of the Cell·C E NabholzA Schneider
Sep 3, 1998·Molecular Biology of the Cell·P J MagalhãesE A Schon
Apr 6, 2004·Molecular Biology of the Cell·Anne Crausaz EsseivaAndré Schneider
Apr 13, 1999·Nucleic Acids Research·S Brubacher-KauffmannA M Duchêne
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Trevor Lithgow, André Schneider
May 16, 2003·Molecular and Cellular Biology·Ludovic DelageLaurence Maréchal-Drouard
Jul 16, 2003·Molecular and Cellular Biology·Subhendra Nath BhattacharyyaSamit Adhya
Dec 4, 2003·Molecular and Cellular Biology·R Lynn SherrerStephen L Hajduk
May 23, 2007·Molekuliarnaia biologiia·P A KamenskiĭI A Tarasov
May 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Srikanta GoswamiSamit Adhya
Aug 5, 2014·Biological & Pharmaceutical Bulletin·Yuri TawarayaHideyoshi Harashima
Feb 16, 2005·FEBS Letters·Marie-Josée LaforestLaurence Maréchal-Drouard
Jun 3, 2008·Trends in Biochemical Sciences·Thalia SalinasLaurence Maréchal-Drouard
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Ludovic DelageLaurence Maréchal-Drouard
Oct 21, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Phong LuCarol S Lim
Oct 26, 1999·The Journal of Biological Chemistry·S MukherjeeS Adhya
Sep 20, 2000·The Journal of Biological Chemistry·S T KapushocL Simpson
Jun 1, 2006·The Journal of Biological Chemistry·Srikanta Goswami, Samit Adhya
Jan 24, 2004·The Journal of Biological Chemistry·Subhendra Nath Bhattacharyya, Samit Adhya
Nov 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Carmine Pasquale CerratoÜlo Langel
Jan 4, 2022·ACS Biomaterials Science & Engineering·Naoto Yoshinaga, Keiji Numata

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