The DC gate in Channelrhodopsin-2: crucial hydrogen bonding interaction between C128 and D156

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Melanie NackJoachim Heberle

Abstract

The light-gated cation channel Channelrhodopsin-2 (ChR2), a retinylidene protein found in the eye-spot of Chlamydomonas reinhardtii, became an optogenetic tool to trigger neurophysiological responses by light and, thus, revolutionized spatio-temporal studies of such processes. The reaction mechanism still remains elusive but recent vibrational spectroscopic experiments started to resolve details of the associated structural changes during the photocycle. Large alterations in the polypeptide backbone were observed by FT-IR spectroscopy that precede and succeed the opening and closing of the channel, respectively. However, the molecular switch that controls gating is still unknown. Here, we present difference spectra of the D156E mutant of ChR2 and assign the observed vibrational bands to crucial hydrogen bonding changes of this residue in various intermediate states of the photoreaction. By comparison with spectra of wild-type ChR2 and the C128T mutant and correlation to electrophysiological studies, we propose the DC gate as a crucial hydrogen-bonding interaction between D156 and C128 which may represent the valve of the channel.

References

Feb 28, 2001·Progress in Biophysics and Molecular Biology·A Barth
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
May 11, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Carsten Kötting, Klaus Gerwert
Jul 27, 2005·Biochemical Society Transactions·G NagelE Bamberg
Nov 13, 2007·The Journal of Biological Chemistry·Oliver P ErnstPeter Hegemann
Nov 27, 2007·Journal of Molecular Biology·Christian BamannErnst Bamberg
May 9, 2009·Journal of the American Chemical Society·Ionela RaduJoachim Heberle
Oct 16, 2009·Current Opinion in Neurobiology·Richard H KramerDirk Trauner
Oct 29, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ionela RaduJoachim Heberle

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Citations

Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Víctor A Lórenz-FonfríaJoachim Heberle
Jan 14, 2012·The Journal of Biological Chemistry·Hiroshi C WatanabeMarcus Elstner
Dec 6, 2011·The Journal of Biological Chemistry·Anna Pia PlazzoMarco Mongillo
Nov 3, 2011·Photochemistry and Photobiology·Sing-Yi HouJohn L Spudich
Feb 27, 2013·The Journal of Biological Chemistry·Eglof RitterFranz J Bartl
Apr 17, 2012·FEBS Letters·Melanie NackJoachim Heberle
Oct 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Víctor A Lórenz-FonfríaJoachim Heberle
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Oct 18, 2011·Journal of Molecular Biology·Maria MüllerWerner Kühlbrandt
Mar 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Nov 21, 2013·Biochimica Et Biophysica Acta·Coral Del ValAna-Nicoleta Bondar
Nov 12, 2013·Biochimica Et Biophysica Acta·Víctor A Lórenz-Fonfría, Joachim Heberle
Mar 1, 2014·Journal of Structural Biology·Coral del ValAna-Nicoleta Bondar
Jul 23, 2015·Biophysical Journal·Tom ReslerJoachim Heberle
Feb 12, 2014·Journal of the American Chemical Society·Shota ItoHideki Kandori
Mar 17, 2017·Annual Review of Biochemistry·Elena G GovorunovaJohn L Spudich
Nov 25, 2017·Science·Oleksandr VolkovValentin Gordeliy
Jan 24, 2012·Nature·Hideaki E KatoOsamu Nureki
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Albert Ardevol, Gerhard Hummer
Sep 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Elena G GovorunovaJohn L Spudich
Feb 7, 2013·Physical Chemistry Chemical Physics : PCCP·Kai WelkeMarcus Elstner
Mar 14, 2020·Biophysics Reviews·Shoko HososhimaSatoshi P Tsunoda
Nov 13, 2020·Nature Communications·Dmitrii ZabelskiiValentin Gordeliy
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Mar 16, 2017·Biophysical Journal·Monika R VanGordonSusan B Rempe
May 22, 2020·The Journal of Physical Chemistry. B·Andrew HarrisLeonid S Brown
Nov 15, 2011·The Journal of Physical Chemistry. B·Kai WelkeMarcus Elstner
Jul 23, 2020·Biophysical Journal·Joel C D KaufmannFranz J Bartl
Mar 30, 2013·Journal of the American Chemical Society·Mirka-Kristin Neumann-VerhoefenJosef Wachtveitl
Oct 28, 2019·Journal of Chemical Theory and Computation·Malte SiemersAna-Nicoleta Bondar
Jan 15, 2015·Journal of the American Chemical Society·Víctor A Lórenz-FonfríaJoachim Heberle
Dec 12, 2018·The Journal of Physical Chemistry Letters·Elena BühlJosef Wachtveitl
Jan 20, 2017·The Journal of Physical Chemistry. B·Tilman KottkeJoachim Heberle

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