The DEAD-box helicase Mss116 plays distinct roles in mitochondrial ribogenesis and mRNA-specific translation

Nucleic Acids Research
Dasmanthie De SilvaAntoni Barrientos

Abstract

Members of the DEAD-box family are often multifunctional proteins involved in several RNA transactions. Among them, yeast Saccharomyces cerevisiae Mss116 participates in mitochondrial intron splicing and, under cold stress, also in mitochondrial transcription elongation. Here, we show that Mss116 interacts with the mitoribosome assembly factor Mrh4, is required for efficient mitoribosome biogenesis, and consequently, maintenance of the overall mitochondrial protein synthesis rate. Additionally, Mss116 is required for efficient COX1 mRNA translation initiation and elongation. Mss116 interacts with a COX1 mRNA-specific translational activator, the pentatricopeptide repeat protein Pet309. In the absence of Mss116, Pet309 is virtually absent, and although mitoribosome loading onto COX1 mRNA can occur, activation of COX1 mRNA translation is impaired. Mutations abolishing the helicase activity of Mss116 do not prevent the interaction of Mss116 with Pet309 but also do not allow COX1 mRNA translation. We propose that Pet309 acts as an adaptor protein for Mss116 action on the COX1 mRNA 5΄-UTR to promote efficient Cox1 synthesis. Overall, we conclude that the different functions of Mss116 in the biogenesis and functioning of the mitochon...Continue Reading

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Citations

Nov 22, 2017·Cell Biology International·Raquel Fonseca Guedes-MonteiroMario H Barros
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Feb 18, 2021·Nature Reviews. Molecular Cell Biology·Eva Kummer, Nenad Ban
Jul 18, 2020·Molecular Cell·Mateusz JaskolowskiNenad Ban
Jun 14, 2021·The Journal of Biological Chemistry·Ying LuoYing Huang
Aug 28, 2021·Cells·Jigeesha Mukhopadhyay, Georg Hausner
Aug 17, 2021·Molecular and Cellular Biology·Jaswinder Kaur, Kaustuv Datta

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Methods Mentioned

BETA
electrophoresis
X-ray
Density Gradient Fractionation
GTPase
Immunoprecipitation
pulldown
co-immunoprecipitation
Y3H

Software Mentioned

Adobe Photoshop
mfold
GrammX
Degenerate finder
quikfold
ITASSER

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