The DEAD-box RNA helicase, Dhh1, functions in mating by regulating Ste12 translation in Saccharomyces cerevisiae

Biochemical and Biophysical Research Communications
Minhan KaJinmi Kim

Abstract

The DEAD-box RNA helicase, Dhh1, is a member of a highly conserved subfamily designated RCK/p54 helicases. Dhh1 functions as mRNA decapping activator, and is localized to discrete cytoplasmic foci known as processing bodies (P-bodies). Here, we describe the essential roles of Dhh1 in the yeast mating pathway. A dhh1 deletion mutation caused a significant decrease in the protein level of Ste12, a mating-specific transcription factor, resulting in severe mating defects. We examined the accumulation of Dhh1-GFP in P-bodies during mating. Following pheromone treatment, the P-body intensity and number increased in wild-type cells, while dhh1 mutant cells failed to show P-body formation. Both the mating and P-body phenotypes of dhh1 were suppressed by overexpression of STE12 or CAF20 encoding an eIF4E inhibitor. In wild-type cells, CAF20 overexpression led to an increased level of Ste12 protein as well as highly developed P-bodies. We propose that Dhh1 and Caf20 regulate the Ste12 protein expression and the Ste12 protein level is associated with P-body formation during mating.

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Citations

Nov 8, 2011·Fungal Genetics and Biology : FG & B·Jong-Hwan Jung, Jinmi Kim
May 3, 2014·Wiley Interdisciplinary Reviews. RNA·Dirk H OstareckAntje Ostareck-Lederer
Mar 27, 2013·Biochimica Et Biophysica Acta·Vlad Presnyak, Jeff Coller
Oct 21, 2009·FEMS Yeast Research·Campbell LouwBenoit Divol
May 15, 2015·PLoS Genetics·Lydia M CastelliGraham D Pavitt
Oct 1, 2015·PLoS Genetics·Virginia BilanchoneSuzanne Sandmeyer
Apr 30, 2017·The Journal of Microbiology·Daehee JungJinmi Kim
Aug 24, 2018·The Journal of Microbiology·Kiyoung ParkJinmi Kim
Jun 25, 2015·Microbiology Spectrum·Suzanne SandmeyerVirginia Bilanchone

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