The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

Viruses
Aleksandra Obrępalska-StęplowskaHenryk Pospieszny

Abstract

Peanut stunt virus (PSV) is a widespread disease infecting legumes. The PSV strains are classified into four subgroups and some are defined by the association of satellite RNAs (satRNAs). In the case of PSV, the presence of satRNAs alters the symptoms of disease in infected plants. In this study, we elucidated the plant response to PSV-G strain, which occurs in natural conditions without satRNA. However, it was found that it might easily acquire satRNA, which exacerbated pathogenesis in Nicotiana benthamiana. To explain the mechanisms underlying PSV infection and symptoms exacerbation caused by satRNA, we carried out transcriptome profiling of N. benthamiana challenged by PSV-G and satRNA using species-specific microarrays. Co-infection of plants with PSV-G + satRNA increased the number of identified differentially expressed genes (DEGs) compared with the number identified in PSV-G-infected plants. In both treatments, the majority of up-regulated DEGs were engaged in translation, ribosome biogenesis, RNA metabolism, and response to stimuli, while the down-regulated DEGs were required for photosynthesis. The presence of satRNA in PSV-G-infected plants caused different trends in expression of DEGs associated with phosphorylation,...Continue Reading

References

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Citations

Sep 24, 2020·Pathogens·Nipin Shrestha, Józef J Bujarski
Oct 21, 2018·International Journal of Molecular Sciences·Barbara WrzesińskaAleksandra Obrępalska-Stęplowska

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Methods Mentioned

BETA
PCR
in vitro transcription
PCRs
Infection
RNA-Seq

Software Mentioned

Relative Expression Software Tool
GOplot
GenePix Pro
blastx
Blast2Go
KEGG Automatic Annotation Server ( KAAS )
Bioconductor limma
R
Blast2GO Pro

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