The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Johanna ArnadottirMartin Chalfie


Gentle touch sensation in Caenorhabditis elegans is mediated by the MEC-4/MEC-10 channel complex, which is expressed exclusively in six touch receptor neurons (TRNs). The complex contains two pore-forming subunits, MEC-4 and MEC-10, as well as the accessory subunits MEC-2, MEC-6, and UNC-24. MEC-4 is essential for channel function, but beyond its role as a pore-forming subunit, the functional contribution of MEC-10 to the channel complex and to touch sensation is unclear. We addressed this question using behavioral assays, in vivo electrophysiological recordings from TRNs, and heterologous expression of mutant MEC-10 isoforms. Animals with a deletion in mec-10 showed only a partial loss of touch sensitivity and a modest decrease in the size of the mechanoreceptor current (MRC). In contrast, five previously identified mec-10 alleles acted as recessive gain-of-function alleles that resulted in complete touch insensitivity. Each of these alleles produced a substantial decrease in MRC size and a shift in the reversal potential in vivo. The latter finding indicates that these mec-10 mutations alter the ionic selectivity of the transduction channel in vivo. All mec-10 mutant animals had properly localized channel complexes, indicatin...Continue Reading


Mar 1, 1981·Developmental Biology·Martin Chalfie, J Sulston
Dec 31, 1997·The Journal of Biological Chemistry·E LinguegliaM Lazdunski
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·Stephan KellenbergerLaurent Schild
Apr 3, 1999·The Journal of Biological Chemistry·S CoscoyM Lazdunski
Jul 10, 1999·The Journal of General Physiology·Stephan KellenbergerLaurent Schild
Sep 25, 1999·The Journal of Biological Chemistry·P M SnyderD B Bucher
Mar 18, 2000·The Journal of Biological Chemistry·S ShengT R Kleyman
Nov 28, 2001·The Journal of General Physiology·Stephan KellenbergerLaurent Schild
Mar 5, 2002·Nature·Miriam B GoodmanMartin Chalfie
Jun 28, 2002·Physiological Reviews·Stephan Kellenberger, Laurent Schild
Jan 14, 2003·Annual Review of Physiology·Miriam B Goodman, Erich M Schwarz
Jun 26, 2003·Science's STKE : Signal Transduction Knowledge Environment·Clay M Armstrong
Jun 28, 2003·Methods : a Companion to Methods in Enzymology·Ravi S Kamath, Julie Ahringer
Jan 1, 2004·The Journal of Biological Chemistry·Mette HesselagerPhilip K Ahring
Jan 31, 2007·The Journal of General Physiology·Austin L BrownMiriam B Goodman
Mar 3, 2007·Bioinformatics·Jason S Papadopoulos, Richa Agarwala
Sep 21, 2007·Nature·Jayasankar JastiEric Gouaux
Oct 23, 2008·Nucleic Acids Research·Sarah HunterCorin Yeats
Jun 1, 2010·Nature Neuroscience·Marios ChatzigeorgiouWilliam R Schafer
Jun 1, 2010·Nature Methods·Andrea CalixtoMartin Chalfie
Oct 1, 2010·Journal of Neurophysiology·Marios ChatzigeorgiouWilliam R Schafer


Apr 20, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Bryan PetzoldBeth L Pruitt
Dec 18, 2013·BMC Neuroscience·Jarred SandersDavid Biron
Jul 17, 2012·Journal of Cell Science·Sergei Sukharev, Frederick Sachs
Feb 18, 2014·Molecular Neurobiology·Yuanzheng Gu, Chen Gu
Oct 3, 2012·Physiology·Amy L Eastwood, Miriam B Goodman
Mar 28, 2012·Molecular Pain·Ramin RaoufJohn N Wood
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Amy L EastwoodMiriam B Goodman
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yushu ChenMartin Chalfie
Nov 14, 2015·Annual Review of Cell and Developmental Biology·Samata KattaMiriam B Goodman
Jun 18, 2015·ELife·Andrés Vidal-GadeaJonathan Pierce-Shimomura
Jun 5, 2014·Biophysical Journal·Richard J PowersAlexander A Spector
Dec 30, 2014·Peptides·Jason C CampbellWilliam G Bendena
Jul 14, 2012·Channels·Philip A Gottlieb, Frederick Sachs
Oct 15, 2013·Cell·Patrick Delmas, Bertrand Coste
Jul 2, 2014·Pflügers Archiv : European journal of physiology·Kate PooleGary R Lewin
Jun 4, 2014·Pflügers Archiv : European journal of physiology·Guy S Bewick, Robert W Banks
Jul 24, 2014·Pflügers Archiv : European journal of physiology·William R Schafer
Apr 8, 2015·PloS One·Bing ShenXiaoqiang Yao
Jun 10, 2016·British Journal of Pharmacology·Emilie BoscardinStephan Kellenberger
Jan 20, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael KriegMiriam B Goodman
Feb 25, 2014·Nature Cell Biology·Michael KriegMiriam B Goodman
Jul 6, 2014·Physiological Reviews·Robert Fettiplace, Kyunghee X Kim
Sep 26, 2014·American Journal of Physiology. Cell Physiology·Rachele SangalettiLaura Bianchi
Sep 20, 2019·The Journal of General Physiology·Samata KattaMiriam B Goodman
Nov 28, 2017·Traffic·Parul SoodSandhya P Koushika
Feb 22, 2020·Annual Review of Neuroscience·Peng JinYuh-Nung Jan
May 18, 2016·The Journal of Biological Chemistry·Shujie ShiT R Kleyman
May 26, 2018·Journal of Biomedical Science·Yuan-Ren ChengChih-Cheng Chen
May 14, 2016·G3 : Genes - Genomes - Genetics·Yushu ChenMartin Chalfie
Mar 2, 2013·G3 : Genes - Genomes - Genetics·Kathleen M ZelleYehuda Ben-Shahar

Related Concepts

MEC-10 protein, C elegans
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