The Degree of Chondral Fragmentation Affects Extracellular Matrix Production in Cartilage Autograft Implantation: An In Vitro Study

Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
Davide Edoardo BonasiaRoberto Rossi

Abstract

To evaluate if the degree of chondral fragmentation affected extracellular matrix (ECM) production in cartilage fragment autograft implantation in vitro. Cartilage was taken from 5 donors undergoing total hip replacement (mean age, 65.6 years; standard deviation [SD], 3). The cartilage was minced to obtain 4 groups with different fragment sizes: (1) "fish scale" (diameter, 8 mm; thickness, 0.3 mm), (2) cubes with 2-mm sides, (3) cubes with 1-mm sides, and (4) cartilage paste (< 0.3 mm). The cultures were maintained in chondrogenic medium for 6 weeks. Biochemically, a proteoglycan (PG):DNA ratio was calculated as the best approximation of ECM production per cell. The ratio between PG released in the culture medium and the PG in the neocartilage (PGrel:PG) was used as a matrix stability index. Histologically, the slides were stained with safranin O fast green and collagen type II immunostaining. The titration of safranin O-positive cells and the Bern score were calculated. Regarding the PG:DNA ratio, group 4 performed significantly better than groups 1 (P = .001) and 3 (P = .02), whereas group 2 performed better than group 1 (P = .03). No significant difference was found regarding the PGrel:PG ratio and safranin O-positive cells....Continue Reading

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Citations

Jun 18, 2019·The American Journal of Sports Medicine·Ryosuke MatsushitaNobuo Adachi
Feb 14, 2020·Cartilage·Bjørn Borsøe ChristensenMartin Lind
Sep 20, 2019·Orthopaedic Journal of Sports Medicine·Clara LevinsonPhilipp Neidenbach
Oct 13, 2017·The American Journal of Sports Medicine·Vonda J WrightJohnny Huard
Mar 24, 2018·Orthopaedic Journal of Sports Medicine·Gian M SalzmannPeter Angele
Feb 2, 2021·Orthopaedic Journal of Sports Medicine·Andrew GudemanJack Farr
May 14, 2021·Arthroscopy Techniques·Klaus Edgar RothGian M Salzmann

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