The degrees of hepatocyte nuclear but not cytoplasmic expression of hepatitis B core antigen reflect the level of viral replication in chronic hepatitis B virus infection.

Journal of Clinical Microbiology
C M ChuY F Liaw

Abstract

Although immunodetection of hepatitis B core antigen (HBcAg) in the liver has long been recognized as a marker of active hepatitis B virus (HBV) replication, the correlation between the level of viral replication and the degrees of expression of HBcAg in hepatocytes remains to be established. In this study, we examined the semiquantitative relationship between the level of HBV DNA in serum and the degree of expression of HBcAg in the hepatocyte nucleus or cytoplasm in 80 adults with chronic hepatitis B. Expression of HBcAg in hepatocytes was studied by the avidin-biotin immunoperoxidase method, and the results were scored on a scale of 0 to 4, values corresponding to the positivity of 0, 1 to 10, 11 to 25, 26 to 50, and > 50%, respectively, of hepatocytes examined. Serum HBV DNA was tested by a liquid hybridization assay, and the results were scored on a scale of 0 to 5, values corresponding to undetectable levels and levels of < or = 50, 51 to 100, 101 to 150, 151 to 200, and > 200 pg/ml, respectively. The results revealed a highly significant, positive correlation between the level of HBV DNA in serum and the degree of expression of HBcAg in nuclei (Spearman rank correlation coefficient [rs] = 0.653, P < 0.001). The mean scor...Continue Reading

References

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Citations

Aug 16, 2005·Alimentary Pharmacology & Therapeutics·H-J YuanC-L Lai
Mar 1, 2008·Hepatology International·William S MasonAllison R Jilbert
Aug 29, 2000·Journal of Clinical Pathology·F ter BorgE A Jones
Jun 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alexander J V ThompsonStephen A Locarnini
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·William S MasonJesse Summers
May 27, 2006·Journal of Gastroenterology and Hepatology·Yusuf UzunOzden Uzunalimoglu
Jan 4, 1998·Journal of Gastroenterology and Hepatology·C M Chu, Y F Liaw
Jan 20, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Rong ZhuHongguang Zhu
Jan 25, 2017·Viruses·Lara Gallucci, Michael Kann
Nov 21, 2007·Journal of Viral Hepatitis·W S MasonA R Jilbert
Dec 7, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Gökhan KabaçamUNKNOWN HIDIT-1 Study Group
Mar 30, 2016·World Journal of Gastroenterology : WJG·Parham SafaieDaryl T Y Lau
Jul 6, 2021·JHEP Reports : Innovation in Hepatology·Jaewon YangMarc Peter Windisch

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