Aug 12, 1976

The demonstration of acid phosphatase in cultured 3T3 mouse cells

Histochemistry
D J BeadleS Amos

Abstract

Acid phosphatase has been demonstrated ultrastructurally in 3T3 and SV40-3T3 mouse cells using sodium beta-glycerophosphate and p-nitrophenyl phosphate as substrate. The former substrate only demonstrates the enzyme in lysosomes and elements of the Golgi apparatus while the latter demonstrates it in the cisternae of the endoplasmic reticulum and in the cell surface as well as at lysosomal sites. The significance of surface acid phosphatase activity is discussed in terms of sublethal autolysis.

Mentioned in this Paper

Acid Phosphatase
Golgi Apparatus
Simian virus 40
Autolysis
4-nitrophenylphosphate
Lysosomes
Beta-glycerol Phosphate
Acid Phosphatase Activity
Lysosomal
Cytochemistry

About this Paper

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