The dengue virus M protein localises to the endoplasmic reticulum and forms oligomers

FEBS Letters
Sook-San WongJ M Mackenzie

Abstract

The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.

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Citations

May 30, 2015·The Journal of General Virology·Claire Scott, Stephen Griffin
Jul 31, 2014·Biochemistry·Henrique Nemésio, José Villalaín
Sep 8, 2018·Frontiers in Immunology·Nagesh K Tripathi, Ambuj Shrivastava
May 22, 2021·European Journal of Medicinal Chemistry·Akshatha H SManisha Singh

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