The dengue virus nonstructural-1 protein (NS1) generates antibodies to common epitopes on human blood clotting, integrin/adhesin proteins and binds to human endothelial cells: potential implications in haemorrhagic fever pathogenesis

Archives of Virology
Andrew Falconar

Abstract

Antibody responses generated by mice to the dengue-2 virus NS1 protein (D-2V NS1) were influenced by MHC class II (I-A) haplotype but each antiserum cross-reacted with human fibrinogen, thrombocytes and endothelial cells. To investigate these findings, a highly avid subclone (MAb 1G5.4-A1-C3) was selected from a parent hybridoma that secreted a monoclonal antibody (MAb) specific for the native dimeric form of D-2V NS1. When MAb reactions were compared using a panel of overlapping synthetic peptides covering the entire protein sequence, dimer specificity was found to be a weak reaction with multiple ELK-type motifs present in either the positive (E/D-hydrophobic-K/R) or negative (K/R-hydrophobic-D/E) orientations. MAb 1G5.4-A1-C3 and highly avid anti-NS1 polyclonal antisera reacted with the NS1 proteins of the four dengue virus serotypes, but only weakly reacted with the NS1 proteins of the other flaviviruses. MAb 1G5.4-A1-C3 and several other anti-NS1 MAbs produced haemorrhage in mice, cross-reacted with human fibrinogen, thrombocytes and endothelial cells, with known epitopes or active sites on human clotting factors and integrin/adhesin proteins present on these cells. D-2V NS1 bound to human endothelial cells via a site with...Continue Reading

Citations

Feb 15, 2001·Journal of Medical Virology·C F LinY S Lin
Sep 11, 2001·Journal of Biomedical Science·H Y LeiC C Liu
Jul 5, 2005·Transactions of the Royal Society of Tropical Medicine and Hygiene·John R Stephenson
Oct 30, 1999·Lancet·N J White
Mar 5, 2003·Antiviral Research·T S GritsunE A Gould
Sep 19, 1998·Clinical and Diagnostic Virology·M A BrintonF A Ennis
Sep 1, 2000·Microbes and Infection·W J McBride, H Bielefeldt-Ohmann
Mar 24, 2010·The Journal of Experimental Medicine·Panisadee AvirutnanJohn P Atkinson
Jul 22, 2010·Vector Borne and Zoonotic Diseases·Ana Carolina Bernardes TerzianMaurício Lacerda Nogueira
Jul 5, 2006·Viral Immunology·Chiou-Feng LinYee-Shin Lin
Sep 21, 2011·The Journal of Infectious Diseases·Anuja MathewAlan L Rothman
Oct 14, 2009·Clinical Microbiology Reviews·Byron E E MartinaAlbert D M E Osterhaus
Jun 8, 2012·Paediatrics and International Child Health·Scott B Halstead
Jun 14, 2013·Journal of Biomedical Science·Shu-Wen WanYee-Shin Lin
Oct 1, 2013·BMC Evolutionary Biology·Boon-Teong TeohSazaly AbuBakar
Aug 24, 2013·BMC Infectious Diseases·Boon-Teong TeohSazaly AbuBakar
Aug 5, 2009·PLoS Neglected Tropical Diseases·Elodie DesclouxXavier De Lamballerie
Jul 14, 2010·PLoS Neglected Tropical Diseases·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
Mar 2, 2011·PLoS Neglected Tropical Diseases·Janet M Doolittle, Shawn M Gomez
Sep 3, 2010·The American Journal of Tropical Medicine and Hygiene·Laurent ThomasRaymond Césaire
May 1, 2013·Future Virology·James S Testa, Ramila Philip

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.