The Density and Length of Filopodia Associate with the Activity of Hyaluronan Synthesis in Tumor Cells

Cancers
Heikki KyykallioKirsi J Rilla

Abstract

Filopodia are multifunctional finger-like plasma membrane protrusions with bundles of actin filaments that exist in virtually all cell types. It has been known for some time that hyaluronan synthesis activity induces filopodial growth. However, because of technical challenges in the studies of these slender and fragile structures, no quantitative analyses have been performed so far to indicate their association with hyaluronan synthesis. In this work we comprehensively address the direct quantification of filopodial traits, covering for the first time length and density measurements in a series of human cancer cell lines with variable levels of hyaluronan synthesis. The synthesis and plasma membrane binding of hyaluronan were manipulated with hyaluronan synthase 3 (HAS3) and hyaluronan receptor CD44 overexpression, and treatments with mannose, 4-methylumbelliferone (4-MU), and glucosamine. The results of this work show that the growth of filopodia was associated with the levels of hyaluronan synthesis but was not dependent on CD44 expression. The results confirm the hypothesis that abundance and length of filopodia in cancer cells is associated with the activity of hyaluronan synthesis.

References

Aug 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W LinR Stern
Dec 18, 2003·The Journal of Biological Chemistry·Aimin Wang, Vincent C Hascall
Jul 2, 2004·Nature Reviews. Cancer·Bryan P Toole
Jul 15, 2005·The Journal of Biological Chemistry·Kirsi RillaRaija H Tammi
Apr 6, 2006·The Journal of Biological Chemistry·Anne KulttiMarkku I Tammi
Oct 3, 2007·Nature Cell Biology·Ekta Seth Chhabra, Henry N Higgs
May 10, 2008·Nature Reviews. Molecular Cell Biology·Pieta K Mattila, Pekka Lappalainen
Jun 25, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kirsi RillaRaija Tammi
Jul 8, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stephen P EvankoThomas N Wight
Jan 9, 2010·Biochimica Et Biophysica Acta·Annette PuschJoachim Kappler
Aug 18, 2010·Communicative & Integrative Biology·Laura M Machesky, Ang Li
Oct 7, 2011·Cell Adhesion & Migration·Seema Khurana, Sudeep P George
Oct 7, 2011·Cell Adhesion & Migration·Antti ArjonenJohanna Ivaska
Jan 11, 2013·The Journal of Biological Chemistry·Kirsi RillaMarkku I Tammi
Jul 25, 2013·BMC Systems Biology·Sharmin NilufarTheodore J Perkins
Feb 5, 2014·The Journal of Cell Biology·Denis TsygankovTimothy C Elston
Jul 18, 2015·Current Opinion in Cell Biology·Guillaume JacquemetJohanna Ivaska
Oct 9, 2015·International Journal of Cell Biology·Kirsi Rilla, Arto Koistinen
Nov 26, 2015·F1000Research·Mary K CowmanAntonio Stecco
Nov 1, 2016·Molecular Biology of the Cell·Matylda RoszkowskaJoanna Dzwonek
Aug 3, 2017·The Journal of Cell Biology·Guillaume JacquemetJohanna Ivaska
Apr 12, 2019·The FEBS Journal·Alberto PassiRenato V Iozzo
May 6, 2019·Cell·Carolyn R ShurerMatthew J Paszek
May 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kayla J WolfSanjay Kumar

❮ Previous
Next ❯

Citations

Apr 4, 2021·International Journal of Molecular Sciences·Sebastian ReiprichVeronika Schönitzer
Oct 7, 2021·Annual Review of Cell and Developmental Biology·Joe Chin-Hun Kuo, Matthew J Paszek

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal
transfections

Software Mentioned

Graph
ADAPT
GraphPad Prism
FiloDetect
MATLAB
CellGeo
ZEN
ImageJ
FiloQuant
- Pad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The American Journal of Pathology
B P Toole, V C Hascall
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Naoki ItanoKoji Kimata
Annals of the Rheumatic Diseases
T C LaurentJ R Fraser
Nature Reviews. Drug Discovery
Sarah Crunkhorn
© 2021 Meta ULC. All rights reserved