The dependence of quorum sensing in Serratia marcescens JG on the transcription of luxS gene

Archives of Microbiology
Shu-Jing SunHu Zhu

Abstract

Bacteria communicate with one another using chemical signal molecules. This phenomenon termed quorum sensing enables the bacteria to monitor the environment for other bacteria and to alter behavior on a population-wide scale in response to cell density. Serratia marcescens JG, a quorum sensing bacterium, can secrete a furanosyl borate diester autoinducer (AI-2) in the exponential phase of growth. In this study, to further investigate the regulation of AI-2 production in S. marcescens JG, the pfs and luxS promoter fusions to an operon luxCDABE reporter were constructed in a low-copy-number vector pBR322K, which allows an examination of transcription of the genes in the pathway for signal synthesis. The results show that the luxS expression is constitutive, and the transcription of luxS is tightly correlated with AI-2 production in S. marcescens JG because the peaks of AI-2 production and transcriptional level of luxS appear at the same time point. The close relation of the profiles of luxS transcription and AI-2 production was also confirmed with real-time PCR technology. These results support the hypothesis that the quorum sensing in S. marcescens JG is luxS dependent.

References

Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R ParsekE P Greenberg
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Jun 12, 2002·Journal of Bacteriology·Anne L Beeston, Michael G Surette
Dec 18, 2002·Journal of Bacteriology·Melanie M MarketonJuan E González
Dec 31, 2003·Advances in Applied Microbiology·Klaus WinzerPaul Williams
Jan 20, 2004·Journal of Bacteriology·Maurizio LabbateStaffan Kjelleberg
Feb 3, 2006·Journal of Bacteriology·Jun-Rong WeiHsin-Chih Lai
Jul 21, 2006·Applied Microbiology and Biotechnology·K E EboigbodinC A Biggs
Nov 14, 2006·Internal Medicine Journal·J McCormack
Apr 27, 2007·FEMS Microbiology Reviews·Rob Van HoudtChris W Michiels
Jul 24, 2008·Annual Review of Biomedical Engineering·Arul Jayaraman, Thomas K Wood
Jun 27, 2009·Molecular Microbiology·Claudia AnetzbergerKirsten Jung
May 22, 2010·Microbiology·L Caetano M AntunesB Brett Finlay
Apr 11, 2012·Bioorganic & Medicinal Chemistry Letters·Dhamodharan BakkiyarajShunmugiah Karutha Pandian
Feb 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jessica Marshall
Feb 18, 2014·Environmental Microbiology·Qian Yang, Tom Defoirdt

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