PMID: 9433473Jan 20, 1998Paper

The dependence of radioimmunoassay detection limits on antibody affinity

Journal of Immunological Methods
T O'Connor, J P Gosling

Abstract

Competitive radioimmunoassay standard curves for progesterone were established with tritiated progesterone and six different anti-progesterone monoclonal antibodies ranging in affinity from 1.23 x 10(8) to 2.82 x 10(10) l/mol. The detection limits for these curves ranged from 133 to 6,670 pmol/l final assay concentration (16.7 to 839.1 pg/tube). Separately, a mass-action mathematical model was used to predict lower detection limits for assays run under equivalent conditions with the six antibodies. When compared to the experimental data the predictions were reliable for the higher affinity antibodies but became less accurate as the affinity decreased.

References

Aug 1, 1976·Acta Endocrinologica·P S BrownM Shahmanesh
Sep 1, 1991·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·H KeilackerM Ziegler
Aug 1, 1990·The Journal of Endocrinology·D J GrovesB A Morris
Jan 1, 1970·Acta Endocrinologica. Supplementum·R Ekins, B Newman
Jun 1, 1971·The Journal of Clinical Endocrinology and Metabolism·K J CattT Tsuruhara
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Apr 12, 1995·Journal of Immunological Methods·A MaresF Kohen
Dec 1, 1994·The Journal of Clinical Investigation·K O KleinG B Cutler
Aug 15, 1996·Analytical Biochemistry·R P Junghans

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Citations

Oct 6, 1998·Journal of Immunological Methods·S J Merrill
Feb 19, 2002·Journal of Immunoassay & Immunochemistry·Jeongeun ChoiMyung Ja Choi
Apr 9, 2009·Journal of Immunological Methods·F HeidebrechtA Bader
Apr 9, 2008·Biophysical Journal·Jody L Swift, David T Cramb
Dec 20, 2014·Journal of Immunoassay & Immunochemistry·A BenabdelazizR Kaidi
May 4, 2005·Analytical Biochemistry·Shiming Lin, Su-Ming Hsu
Jul 15, 2018·Chemistry : a European Journal·Hyeongjoo Choi, Yongwon Jung
Dec 31, 2019·Journal of Immunoassay & Immunochemistry·Aldjia BenabdelazizRachid Kaidi
Mar 11, 2018·Biochemistry. Biokhimii︠a︡·D V SotnikovB B Dzantiev
Dec 29, 2020·Cancer Control : Journal of the Moffitt Cancer Center·Hao ChenShuqin Dai
Jun 11, 1999·Analytical Biochemistry·M A Model, K E Healy

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