The deregulation of miR-17/CCND1 axis during neuroendocrine transdifferentiation of LNCaP prostate cancer cells

PloS One
Jaroslaw Thomas DankertGunther Wennemuth

Abstract

Prostate carcinoma contain foci of neuroendocrine transdifferentiation, resulting in an increase of androgen-independent neuroendocrine-like (NE) tumor cells, whose number significantly correlates with tumor aggressiveness and thus lower survival rate. Neuroendocrine transdifferentiation of prostate cancer cells and a potential role of miRNAs within this process are poorly understood. MicroRNAs are small non-coding RNAs which post-transcriptionally regulate gene expression. The aim of this project was to identify new genes and miRNAs involved in neuroendocrine transdifferentiation. LNCaP prostate cancer cells were differentiated to NE-like cancer cells and microarray analyses were performed. Microarray results have been validated for the eight most deregulated mRNAs and microRNAs via qRT-PCR and analyzed with different algorithms to predict new targets for deregulated microRNAs. The induced CyclinD1 gene could be validated as new target gene for the repressed miR-17 family containing miR-17, miR-20a, miR-20b, miR-106a and miR-106b via reporter gene assays and Western Blot. Functional analysis of miR-17 family shows a high influence on cell proliferation, colony forming ability and apoptosis in LNCaP cells. Our data demonstrate ...Continue Reading

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Citations

Jun 20, 2019·Cellular and Molecular Life Sciences : CMLS·Delin KongChun-Bo Teng
Jul 17, 2019·Journal of Cellular Physiology·Mingming WangXiangdong Li
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dawid MehlichPaweł K Włodarski

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Methods Mentioned

BETA
FCS
Chips
Chip
PCR
transfection
electrophoresis
gene array
nucleotide exchange
FACS

Software Mentioned

Cell Quest
Affymetrix GeneChip Operating Software
Affymetrix
DAVID
TargetScan
ImageJ

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