The derivation of mesenchymal stem cells from human embryonic stem cells.

Cells, Tissues, Organs
Shelley E BrownPaul H Krebsbach

Abstract

Human embryonic stem cells (hESCs) hold promise for tissue regeneration therapies by providing a potentially unlimited source of cells capable of undergoing differentiation into specified cell types. Several preclinical studies and a few clinical studies use human bone marrow stromal cells (hBMSCs) to treat skeletal diseases and repair damaged tissue. However, hBMSCs have limited proliferation and differentiation capacity, suggesting that an alternate cell source is desirable, and hESCs may serve this purpose. Here we describe a protocol for the reproducible derivation of mesenchymal stem cells from hESCs (hES-MSCs). The hES-MSCs have a similar immunophenotype to hBMSCs, specifically they are CD73+, STRO-1+ and CD45-, and are karyotypically stable. The derived hES-MSCs are also capable of differentiating into osteoblasts and adipocytes. When the hES-MSCs were genetically modified with the lineage-specific Col2.3-GFP lentivirus and cultured in osteogenic medium, increased GFP expression was detected over time, indicating the hES-MSCs have the capacity to differentiate down the osteogenic lineage and had progressed toward a mature osteoblast phenotype.

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Citations

Sep 20, 2008·Chemical Reviews·Adele L Boskey, Rani Roy
Aug 12, 2010·Cellular Reprogramming·Premjit ArpornmaeklongPaul H Krebsbach
May 23, 2012·Stem Cells and Development·Amer MahmoodMoustapha Kassem
Apr 3, 2014·Archives of Toxicology·Walid ZaherMoustapha Kassem
Nov 12, 2013·Tissue Engineering. Part B, Reviews·Siyeon Park, Gun-Il Im
May 19, 2009·Expert Review of Anticancer Therapy·Thomas KosztowskiAlfredo Quiñones-Hinojosa
Jan 22, 2010·Expert Opinion on Biological Therapy·Zhongmin ZouHuiqin Sun
Jan 19, 2013·Expert Opinion on Biological Therapy·Zhanpeng ZhangPeter X Ma
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Nov 16, 2010·Pharmacology & Therapeutics·Phillip CallihanShelley B Hooks
Aug 13, 2013·Journal of Dental Research·X QianP H Krebsbach
Feb 20, 2018·Tissue Engineering. Part B, Reviews·Sarita KumariDeepa Subramanyam
Sep 27, 2014·World Journal of Stem Cells·Anne-Laure Hafner, Christian Dani
May 11, 2019·Orthodontics & Craniofacial Research·Zeena QiryaqozLuis G Villa-Diaz
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Jun 7, 2019·World Journal of Stem Cells·Soo Kim, Tae Min Kim
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wai Long TamScott J Roberts

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