The detection and mapping of the spatial distribution of insect defense compounds by desorption atmospheric pressure photoionization Orbitrap mass spectrometry

Analytica Chimica Acta
Jan RejšekJosef Cvačka

Abstract

Many insects use chemicals synthesized in exocrine glands and stored in reservoirs to protect themselves. Two chemically defended insects were used as models for the development of a new rapid analytical method based on desorption atmospheric pressure photoionization-mass spectrometry (DAPPI-MS). The distribution of defensive chemicals on the insect body surface was studied. Since these chemicals are predominantly nonpolar, DAPPI was a suitable analytical method. Repeatability of DAPPI-MS signals and effects related to non-planarity and roughness of samples were investigated using acrylic sheets uniformly covered with an analyte. After that, analytical figures of merit of the technique were determined. The spatial distribution of (E)-1-nitropentadec-1-ene, a toxic nitro compound synthesized by soldiers of the termite Prorhinotermes simplex, was investigated. Then, the spatial distribution of the unsaturated aldehydes (E)-hex-2-enal, (E)-4-oxohex-2-enal, (E)-oct-2-enal, (E,E)-deca-2,4-dienal and (E)-dec-2-enal was monitored in the stink bug Graphosoma lineatum. Chemicals present on the body surface were scanned along the median line of the insect from the head to the abdomen and vice versa, employing either the MS or MS(2) mode....Continue Reading

References

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Apr 15, 2010·Rapid Communications in Mass Spectrometry : RCM·Laura LuosujärviTiina J Kauppila
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Citations

Jan 19, 2016·Rapid Communications in Mass Spectrometry : RCM·T J KauppilaR A Ketola
Mar 29, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Jana HavlíčkováRobert Hanus
Nov 2, 2019·Archives of Insect Biochemistry and Physiology·Fei-Ying YangMin-Sheng You
Oct 5, 2017·Analytical Chemistry·Jan RejšekJosef Cvačka

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